Modification of post-industrial lignin by fungal strains of the genus Trichoderma isolated from different composting stages
Modification of post-industrial lignin by fungal strains of the genus Trichoderma isolated from different composting stages
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DOI：
10.1016/j.jenvman.2020.110573
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发表时间：
2020-07-15
影响因子：
8.7
通讯作者：
Kornillowicz-Kowalska, Teresa
中科院分区：
文献类型：
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作者：
Bohacz, Justyna;Kornillowicz-Kowalska, Teresa
环境管理链中的一个环节是对新兴的工业后废物进行环境友好利用，并改进其处理方法。本研究的目的和创新之处在于评价真菌净化含后工业木质素废水的潜力，即来自制浆造纸工业的废水。木霉在不同质量和数量的木质纤维化合物堆肥中占主导地位。研究中使用的木霉菌株是在三个不同的时间点从两个木质纤维堆肥中分离出来的(分别来自10周、20周和30周的堆肥)。对木霉属的18个菌株进行了降解0.2%工业后木质素的能力测试。通过测定脱色率、木质素分解酶活力和培养后液中酚类化合物的浓度对其进行评价。从10周龄堆肥I和30周龄堆肥II中分离到的木霉菌株对暗后工业木质素的脱色活性和生物转化能力最高。所有菌株都能分泌类辣根过氧化物酶(HRP-like)、类超氧化物歧化酶(SOD-like)、木聚糖酶和酚类化合物。从30周堆肥I和10周堆肥II分离出的菌株向含有后工业木质素的培养液中释放出最多的酚类化合物。从10周和20周堆肥中分离到的菌株分别具有较高的类超氧化物歧化酶和类HRP活性。用高效液相色谱法测定堆肥I中木霉菌VII培养物中酚类化合物的浓度与其脱色程度和较高的HRP活性相一致。研究结果表明，从堆肥初期分离到的具有脱色活性的木霉菌可用于工业木质素废弃物的生物转化。
One of the links in the environmental management chain is the environmentally friendly utilization of the emerging post-industrial waste and improvement of the methods of processing thereof. The aim and novelty of this research was to evaluate the potential of fungi to purify wastewater containing post-industrial lignin, i.e. waste originating from the pulp and paper industry. Trichoderma were dominant in the composts with different qualities and quantities of lignocellulosic compounds. The Trichoderma strains used in the research were isolated from two lignocellulosic composts at three different time points (from 10-, 20- and 30-week-old composting mass). Eighteen strains of the genus Trichoderma were tested for their ability to biodegrade 0.2% post-industrial lignin. It was evaluated by determination of decolorization, activities of ligninolytic enzymes, and concentration of phenolic compounds in the post-culture liquid. The Trichoderma strains isolated from 10-week-old compost I and 30-week-old compost II showed the highest decolorization activity and biotransformation of dark post-industrial lignin. All strains secreted horseradish-like peroxidase (HRP-like), superoxide dismutase-like (SOD-like), xylanase, and phenolic compounds. Strains isolated from 30-week-old compost I and from 10-week-old compost II released the greatest amounts of phenolic compounds into the culture liquid containing post-industrial lignin. The strains isolated from 10- and 20-week-old compost were characterized by high SOD-like and HRP-like activity, respectively. The concentration of phenolic compounds measured with HPLC in Trichoderma fungus culture VII from compost I corresponded with the decolorization degree and high HRP-like activity. The study results indicate that the genus Trichoderma with decolorization activity isolated from the first composting stages can be used in the biotransformation of post-industrial lignin waste.