Integrate Small RNA and Degradome Sequencing to Reveal Drought Memory Response in Wheat (Triticum aestivum L.).
Integrate Small RNA and Degradome Sequencing to Reveal Drought Memory Response in Wheat (Triticum aestivum L.).
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整合小RNA和降解组测序揭示小麦干旱记忆反应
DOI：
10.3390/ijms23115917
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发表时间：
2022-05-25
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
干旱已逐渐成为植物最严重的非生物胁迫之一。经历应激训练的植物可以表现出增强的应激耐受性。根据MicroRNA（miRNA）测序数据，本研究共鉴定出195个小麦干旱记忆相关的候选miRNAs，其中64个（32.8%）候选miRNAs的靶标通过degradome测序得到验证。几种干旱记忆相关的miRNAs如tae-miR 9676 - 5 p、tae-miR 9676-p3_1ss21GA、tae-miR 171 a、tae-miR 531_L-2、tae-miR 408_L-1、PC-3 p-5049_3565、tae-miR 396 c-5 p、tae-miR 9778、tae-miR 164 a-5 p、和tae-miR 9662 a-3 p通过调节其靶基因的表达而被验证为对干旱记忆具有强响应。此外，过量表达与干旱记忆相关的miR 531_L-2可以显著提高转基因拟南芥的耐旱性。干旱记忆可以通过转录记忆调节植物细胞信号转导、植物生物合成等生物学过程来科普干旱。此外，干旱记忆相关的miRNAs还可以促进淀粉、蔗糖和可溶性糖的积累，调节脯氨酸的稳态，从而提高植物的抗旱性。研究结果有助于理解小麦幼苗的干旱记忆，并为抗旱育种提供新的策略。
Drought has gradually become one of the most severe abiotic stresses on plants. Plants that experience stress training can exhibit enhanced stress tolerance. According to MicroRNA (miRNA) sequencing data, this study identified 195 candidate drought memory-related miRNAs in wheat, and targets of 64 (32.8%) candidate miRNAs were validated by degradome sequencing. Several drought memory-related miRNAs such as tae-miR9676-5p, tae-MIR9676-p3_1ss21GA, tae-miR171a, tae-miR531_L-2, tae-miR408_L-1, PC-3p-5049_3565, tae-miR396c-5p, tae-miR9778, tae-miR164a-5p, and tae-miR9662a-3p were validated as having a strong response to drought memory by regulating the expression of their target genes. In addition, overexpression of drought memory-related miRNA, tae-miR531_L-2, can remarkably improve the drought tolerance of transgenic Arabidopsis thaliana. Drought memory can regulate plant cellular signal transduction, plant biosynthetic processes, and other biological processes to cope with drought via transcriptional memory. In addition, drought memory-related miRNAs can promote starch and sucrose catabolism and soluble sugar accumulation and regulate proline homeostasis to improve plant drought resistance. Our results could contribute to an understanding of drought memory in wheat seedlings and may provide a new strategy for drought-resistant breeding.
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影响因子：
4.3
作者：
Balibrea, ME;RusAlvarez, AM;PerezAlfocea, F
通讯作者：
PerezAlfocea, F
影响因子：
3.7
作者：
Fleta-Soriano E;Pintó-Marijuan M;Munné-Bosch S
通讯作者：
Munné-Bosch S
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影响因子：
5.7
作者：
Abid, Muhammad;Hakeem, Abdul;Dai, Tingbo
通讯作者：
Dai, Tingbo
影响因子：
9.2
作者：
Addo-Quaye, Charles;Eshoo, Tifani W.;Axtell, Michael J.
通讯作者：
Axtell, Michael J.
影响因子：
5.8
作者：
Addo-Quaye, Charles;Miller, Webb;Axtell, Michael J.
通讯作者：
Axtell, Michael J.