Saccharospirillum mangrovi sp nov., a bacterium isolated from mangrove sediment
Saccharospirillum mangrovi sp nov., a bacterium isolated from mangrove sediment
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Saccharospirillum mangrovi sp nov.，一种从红树林沉积物中分离出来的细菌
DOI：
10.1099/ijsem.0.002899
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发表时间：
2018
影响因子：
2.8
通讯作者：
He Shan
中科院分区：
文献类型：
--
作者：
Zhang Weiyan;Yuan Ye;Su Dengquan;Ding Lijian;Yan Xiaojun;Wu Min;Epstein Slava S.;He Shan
从海南省海口市红树林沉积物样品中分离到一株革兰氏阴性、螺旋菌、无芽胞、游动、严格需氧细菌，定名为HK-33T。菌株HK-33T能在10~45 °C(最适37 °C)、0.5~12.0 %(w/v)、NaC(2.0 %，w/v)、pH 5.5~8.5(pH 7.0)条件下生长。主要的细胞脂肪酸是C16 ： 0和总和特征8(C18 ： 1ω7c和/或C18 ： 1ω6c)。泛苯二酚-8是主要的呼吸对苯二酚，Q-9有微量存在。极性脂包括二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、两种未鉴定的磷脂、两种未鉴定的糖脂、一种未鉴定的氨基磷脂和两种未鉴定的脂。DNAG+C 含量为57.3mol% 。根据16S rRNA基因序列的相似性，HK-33T菌株与蔗糖螺菌CPA1T、不耐盐糖螺菌EL-105T、盐生糖螺菌YIM-Y25T和甘蔗螺菌IMCC 4453T的序列相似性分别为98.6 %、96.4%、95.7%和94.9 %。系统发育分析表明，HK-33T菌株与S.CpA1T、不耐寒链球菌EL-105T、盐生链球菌YIM-Y25T和S.AestuariiIMCC 4453T。DNA-DNA杂交分析结果表明，HK-33T株的DNA同源性为36.3±1.7 %。相关的CPA1T。根据其表型、化学分类和基因分型特征，HK-33T菌株被认为是甘蔗螺旋菌属中的一个新种，命名为Saccharspirillomgrovisp。11月是提议的。模式菌株为HK-33T(=KCTC 62178T=MCCC 1K03440T)。
A Gram-negative, spirilla, non-spore-forming, motile and strictly aerobic bacterium, designated HK-33T, was isolated from a mangrove sediment sample in Haikou city, Hainan Province, China. Strain HK-33Twas able to grow at 10–45 °C (optimum 37 °C), 0.5–12.0 % (w/v) NaCl (2.0 %, w/v) and pH 5.5–8.5 (pH 7.0). The major cellular fatty acids were C16 : 0and summed feature 8 (C18 : 1ω7cand/or C18 : 1ω6c). Ubiquinone-8 was the predominant respiratory quinone, and Q-9 was present in trace amounts. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids, two unidentified glycolipids, an unidentified aminophosphoglycolipid and two unidentified lipids. The DNA G+C content was 57.3 mol%. According to 16S rRNA gene sequences similarity, strain HK-33Tshared 98.6 %, 96.4%, 95.7 and 94.9 % sequence similarities to the speciesSaccharospirillum correiaeCPA1T,Saccharospirillum impatiensEL-105T,Saccharospirillum salsuginisYIM-Y25TandSaccharospirillum aestuariiIMCC 4453T, respectively. Phylogenetic analysis showed that strain HK-33Twas clustered withS. correiaeCPA1T,S. impatiensEL-105T,S. salsuginisYIM-Y25TandS. aestuariiIMCC 4453T. Results of DNA–DNA hybridization analysis revealed that strain HK-33Tshared 36.3±1.7 % DNA relatedness withS. correiaeCPA1T. On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain HK-33Tis considered to represent a novel species in the genusSaccharospirillum, for which the nameSaccharospirillummangrovisp. nov. is proposed. The type strain is HK-33T(=KCTC 62178T=MCCC 1K03440T).