Crypt cell antigen expression in human colon tumor cell lines: analysis with a panel of monoclonal antibodies to CaCo-2 luminal membrane components.
Crypt cell antigen expression in human colon tumor cell lines: analysis with a panel of monoclonal antibodies to CaCo-2 luminal membrane components.
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人结肠肿瘤细胞系中的隐窝细胞抗原表达：使用一组针对 CaCo-2 腔膜成分的单克隆抗体进行分析。
DOI：
10.1093/jnci/76.4.571
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Quaroni,A
中科院分区：
文献类型：
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作者：
Quaroni,A
用20株BALB/c小鼠抗CaCo-2细胞表面膜成分的单克隆抗体，研究了培养的人结肠癌细胞中小肠标志物的表达。这些抗体中的15个识别的抗原被鉴定为相对高分子量的膜蛋白，其中之一对应于蔗糖酶-异麦芽糖酶的单链前体。用免疫荧光法，发现14种抗体可染色小肠上皮细胞，并观察到人空肠中各种不同的抗原分布模式。5种抗体仅对隐窝细胞染色，3种抗体仅对吸收绒毛细胞的腔膜染色，4种抗体仅对整个上皮染色。相反，只有4种抗体染色正常人结肠组织。由这组单克隆抗体定义的不同抗原在测试的14个人肠肿瘤细胞系中的表达中显示出很大的变异性。这些结果证明了许多结肠肿瘤细胞系和肠隐窝细胞之间惊人的相似性，并确定了一组新的肠细胞表面标志物，可用于在体内和培养中鉴定和表征肠肿瘤细胞。
Twenty BALB/c mouse monoclonal antibodies to surface membrane components of confluent CaCo-2 cells were used to study the expression of small intestine markers in cultured human colon tumor cells. The antigens recognized by 15 of these antibodies were identified as membrane proteins of relatively high molecular weight, one of them corresponding to the single-chain precursor of sucrase-isomaltase. By immunofluorescence, 14 antibodies were found to stain the small intestine epithelial cells, and various distinctive patterns of antigen distribution in human jejunum were observed. Five antibodies stained exclusively the crypt cells, 3 the luminal membranes of the absorptive villus cells, and 4 the entire epithelium. In contrast, only 4 antibodies stained normal human colon tissue. The different antigens defined by this panel of monoclonal antibodies showed great variability in their expression in 14 human intestinal tumor cell lines tested. These results demonstrated a surprising similarity between many colon tumor cell lines and intestinal crypt cells and identified a set of new intestinal cell surface markers that can be used to identify and characterize intestinal tumor cells in vivo and in culture.