STUDIES ON THE CRYSTALLINE LENS: I. Technic for in Vitro Culture of Crystalline Lenses and Observations on Metabolism of the Lens
STUDIES ON THE CRYSTALLINE LENS: I. Technic for in Vitro Culture of Crystalline Lenses and Observations on Metabolism of the Lens
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晶状体的研究：一、晶状体体外培养技术及晶状体代谢观察
DOI：
10.1001/archopht.1950.00910010996003
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发表时间：
1950
影响因子：
--
通讯作者：
V. Kinsey
中科院分区：
文献类型：
--
作者：
F. Merriam;V. Kinsey
需要一种体外培养晶状体的方法，该方法适用于在12至24小时内进行定量代谢研究，这引起了本研究。在透镜上进行实验的可能性显然是无限的，如果有可能在相对不确定的时间内将透镜保持在正常的生理状态下，则刺激我们使用不同的培养基进行几次初步实验，试图建立这样的条件。本文描述了一种培养晶状体的技术，以及观察在不同培养基中培养约一周的晶状体所经历的碳水化合物代谢的变化，碳水化合物代谢是损伤的敏感指标。以前培养晶状体的方法是基于de Haan发明的灌注技术。1例如，Bakker将兔晶状体置于一个浅的气密和水密的玻璃室中，然后用无菌的
THE NEED for an in vitro method of cultivating lenses which would be suitable for performing quantitative metabolic studies over periods of twelve to twenty-four hours gave rise to the present investigation. The apparently limitless possibilities for experimentation on the lens, were it possible to maintain lenses in a normal physiologic state for a relatively indefinite period, stimulated us to perform several preliminary experiments, using different culture mediums, in an attempt to establish such conditions. In this paper a technic is described for culturing lenses, as well as observations on changes in carbohydrate metabolism—a sensitive index of damage—which lenses undergo when incubated in different mediums for periods of approximately a week. Previous methods of culturing lenses have been based on a perfusion technic devised by de Haan.1Bakker,2for example, placed rabbit lenses in a shallow air-tight and water-tight glass chamber and then perfused the lenses with sterile