In vitro brain slice studies of the rat's dorsal nucleus of the lateral lemniscus. II. Physiological properties of biocytin-labeled neurons.
In vitro brain slice studies of the rat's dorsal nucleus of the lateral lemniscus. II. Physiological properties of biocytin-labeled neurons.
复制标题
大鼠外侧丘系背核的体外脑切片研究。
DOI：
10.1152/jn.1995.73.2.794
复制
发表时间：
1995
影响因子：
2.5
通讯作者：
J. Kelly
中科院分区：
文献类型：
--
作者：
S. Wu;J. Kelly
1.对大鼠外侧丘系背核(DNLL)神经元进行细胞内记录，测定其固有和突触的生理特性，并用细胞内注射生物细胞素法进行标记。生物细胞素标记的神经元被重建，并根据其体细胞和树突的形态进行分类。2.鉴定了DNLL细胞形态类型的多样性。识别出五个主要的神经元群：多极；细长的I、II和III；以及圆形。多极细胞的特征是几个大的树突，多个分支，分布在DNLL内的大片区域。树枝状物向各个方向均匀辐射。3.细长细胞以伸展的胞体为特征，胞体呈极性树突。在细长的I和II细胞中，树突优先定向在水平面上，树突分支从内侧向外侧延伸到DNLL的大部分细胞构筑宽度。细长II的分类保留给单个神经元，这些神经元具有丰富的树突分支，沿着DNLL的边缘呈扇形展开。这种神经元在我们的样本中是独一无二的，不同于更常见的细长I细胞，后者具有较少的背腹侧树突分支。细长的III细胞有延伸的胞体，但其树突没有延伸到DNLL上，也没有显示出择优取向。4.圆形神经元胞体较小，胞体呈圆形，胞体呈放射状，树突呈放射状，分布于DNLL内。这些细胞在我们的样本中相当常见，几乎可以肯定与尼氏染色切片中罕见的小圆形细胞不同。一些生物细胞素标记的神经元很难分类为多极、细长的I、II或III，或圆形。这些神经元具有与细长III细胞最相似的特性，但这里将它们分开处理，以最大限度地减少形态类别中的异质性。5.本研究测量的固有生理特性与DNLL神经元的形态分类无关。所有DNLL神经元都有相似的电流-电压曲线，无论其解剖类型如何。细胞内注入正电流产生一系列持续的动作电位，其数目与电流注入的大小有关。虽然一些细胞有随着电流注入时间的延长而增加或减少间期长度的趋势，但棘波间期是规则的。虽然一些细胞有随着电流注入时间的延长而增加或减少间期长度的趋势，但棘波间期是规则的。注入负电流会产生与电流强度成正比的超极化。
1. We made intracellular recordings from neurons in rat dorsal nucleus of the lateral lemniscus (DNLL), determined intrinsic and synaptic physiological properties, and labeled the cells by intracellular injection of biocytin. Biocytin-labeled neurons were reconstructed and classified according to their somatic and dendritic morphology. 2. We identified a diversity of morphological cell types in DNLL. Five main groups of neurons were recognized: multipolar; elongate I, II, and III; and round. The multipolar cells were characterized by several large dendrites with multiple branches that spread over large areas within the DNLL. The dendrites radiated equally in all directions. 3. Elongate cells were characterized by extended cell bodies with polar dendrites. In the case of elongate I and II cells, the dendrites were preferentially oriented in the horizontal plane and the dendritic branches extended across most of the cytoarchitectonic breadth of DNLL from the medial to lateral borders. The classification of elongate II was reserved for a single neuron with profuse dendritic branching that fanned out dorsoventrally along the margins of DNLL. This neuron was unique in our sample and was distinguished from the more common elongate I cells, which had less profuse dorsoventral dendritic branching. Elongate III cells had extended cell bodies, but their dendrites did not extend across the DNLL and showed no preferential orientation. 4. Round neurons had relatively small, round cell bodies and radial dendrites that extended over large areas within DNLL. These cells were quite common in our sample and are almost certainly not the same as the infrequently encountered small round cells found in Nissl-stained sections. Some biocytin-labeled neurons were difficult to classify as either multipolar, elongate I, II, or III, or round. These neurons had properties that most closely resembled elongate III cells, but they were treated separately here to minimize heterogeneity within morphological categories. 5. The intrinsic physiological properties measured in this study were uncorrelated with the morphological class of DNLL neurons. All DNLL neurons had similar current-voltage curves regardless of their anatomic category. Intracellular injection of positive current produced a sustained series of action potentials, the number of which was related to the magnitude of current injection. The interspike intervals were regular, although some cells had a tendency toward an increase or decrease in the length of the interval with prolonged current injection. The interspike intervals were regular, although some cells had a tendency toward an increase or decrease in the length of the interval with prolonged current injection. Injection of negative current produced a hyperpolarization that was proportional to the current strength.(ABSTRACT TRUNCATED AT 400 WORDS)