A novel 31-amino-acid-length endothelin, ET-1(1-31), can act as a biologically active peptide for vascular smooth muscle cells.
A novel 31-amino-acid-length endothelin, ET-1(1-31), can act as a biologically active peptide for vascular smooth muscle cells.
复制标题
一种新型的 31 个氨基酸长度的内皮素 ET-1(1-31)，可以作为血管平滑肌细胞的生物活性肽。
DOI：
10.1006/bbrc.2000.3292
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发表时间：
2000
影响因子：
3.1
通讯作者：
Yutaka Nakaya
中科院分区：
文献类型：
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作者：
Noriko Nagata;Y. Niwa;Yutaka Nakaya
人凝乳酶产生一种新的内皮素-1，它比传统的ET-1，ET-1(1-21)长，有31个氨基酸的长度。本研究旨在探讨ET-1(1-31)在猪冠状动脉血管平滑肌细胞(VSMC)中的作用。ET-1(1-31)对VSMC增殖、c-fos/c-myc基因表达和细胞周期分析的作用与ET-1(1-21)相当，但ET-1(1-31)对[Ca(2+)](I)的增加作用比ET-1(1-21)弱10倍。ET-1(1-31)可显著诱导细胞周期蛋白D1的表达，但不能诱导细胞周期蛋白D2和D3的表达。这些作用可被ET(A)受体拮抗剂BQ485特异性抑制，但ET-1(1-21)不是BQ485所特有的，提示ET-1(1-21)与ET-1(1-21)具有不同的受体特异性。这些结果表明，ET-1(1-31)与ET-1(1-21)相比具有较弱的血管收缩能力和不同的VSMC受体亚型，但也可参与VSMC的增殖过程，如动脉粥样硬化。
Human chymase produces a novel endothelin-1 with 31 amino-acid length ¿ET-1(1-31)¿, which is longer than conventional ET-1, ¿ET-1(1-21)¿. The aim of our study was to investigate the role of ET-1(1-31) on porcine coronary vascular smooth muscle cell (VSMC). Although the increase in [Ca(2+)](i) by ET-1(1-31) was 10 times weaker than that of ET-1(1-21), ET-1(1-31) showed equivalent potency in VSMC proliferation, c-fos/c-myc mRNA expression and cell cycle analysis with ET-1(1-21). ET-1(1-31) significantly induced expression of cyclin D1 but not those of cyclin D2 or D3. These effects were specifically inhibited by BQ485, an ET(A) receptor antagonist, although that of ET-1(1-21) was not specific to BQ485, suggesting different receptor specificity from ET-1(1-21). These results indicate that ET-1(1-31) also can involve a VSMC proliferation process such as atherosclerosis, although it has weaker vasoconstricting potency and different receptor subtypes on VSMC from those of ET-1(1-21).