Novel source of resistance to tomato yellow leaf curl virus exhibiting a symptomless reaction to viral infection
Novel source of resistance to tomato yellow leaf curl virus exhibiting a symptomless reaction to viral infection
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DOI：
10.21273/jashs.123.6.1004
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发表时间：
1998-11-01
影响因子：
1.9
通讯作者：
Pilowsky, M
中科院分区：
文献类型：
--
作者：
Friedmann, M;Lapidot, M;Pilowsky, M
番茄黄卷叶病毒（TYLCV）是由烟粉虱（Bemisia tabaci Genn.）传播的，对热带和亚热带地区的番茄（Lycopersicon esculentum L.）作物具有破坏性。培育抗病品种是控制TYLCV的最佳选择。然而，在Volcani中心测试的所有可用的抗性商业品种，当接种TYLCV时，都出现了不同程度的疾病症状。在本研究中，我们报道了一种TY172系，TY172系是TYLCV的无症状携带者，无论是在温室中被毒粉虱感染，还是在自然感染下在田间生长，TY172系都没有出现疾病症状。在受感染的TY172植株中检测到病毒DNA，尽管其水平远低于易感感染对照。此外，将受感染的易感接穗嫁接到TY172砧木上的嫁接实验表明，即使连续暴露于非常高水平的病毒中，TY172也没有出现疾病症状，也没有积累高水平的病毒。将TY172与感感亲本杂交时，杂种表现出比感感亲本更温和的症状和更低的病毒含量，但高于TY172，表明TY172的抗性部分优势。接种F-2人群后，出现无症状个体的比例约为7:64。这表明至少有三个基因可以解释这种抗性。
Tomato yellow leaf curl virus (TYLCV), transmitted by the tobacco whitefy (Bemisia tabaci Genn.), can be devastating to tomato (Lycopersicon esculentum L.) crops in tropical and subtropical regions. The development of resistant cultivars is the best option for control of TYLCV. However, all the available resistant commercial cultivars tested at the Volcani Center, when inoculated with TYLCV, developed different levels of disease symptoms. In this study, we report the development of a breeding line, TY172, which is a symptomless carrier of TYLCV, Line TY172, whether infected in the greenhouse with viruliferous whiteflies, or when grown in the field under natural infection, showed no symptoms of the disease. Viral DNA was detected in infected TY172 plants, albeit at much lower levels than a susceptible infected control. In addition, grafting experiments using infected susceptible scions grafted onto TY172 stocks, showed that even when exposed continuously to very high levels of virus, line TY172 did not develop disease symptoms, nor did it accumulate high levels of the virus. When TY172 was crossed with susceptible lines, the hybrids exhibited milder symptoms and lower viral content than the susceptible parent, yet higher than that of TY172, suggesting a partial dominance for the TY172 resistance. Upon inoculation of F-2 populations, the amount of symptomless individuals appeared in a ratio of approximate to 7:64. This suggests that at least three genes may account for the resistance.