Aging-related differences in cerebral capillary blood flow in anesthetized rats
Aging-related differences in cerebral capillary blood flow in anesthetized rats
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DOI：
10.1016/j.neurobiolaging.2014.01.136
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发表时间：
2014-08-01
影响因子：
4.2
通讯作者：
Lesage, Frederic
中科院分区：
文献类型：
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作者：
Desjardins, Michele;Berti, Romain;Lesage, Frederic
已经用各种成像方式测量了基线脑血流量的脑血管病相关减少，然而，毛细血管流动对这种现象的贡献仍有待阐明。本研究使用双光子激光扫描荧光显微镜测量毛细血管直径，红细胞的速度，并在个别毛细血管的感觉运动皮层的12个成年（3个月大）和12个老年（24个月大）的雄性Long-Evans大鼠异氟烷麻醉下的流量。921个毛细管的平均（+/-标准差）直径和速度分别为6.4 +/- 1.4 μ m和1.3 +/- 1.1 mm/s。与年轻动物相比，老年动物的红细胞速度和流量分别显著增加了48%和15%（p < 5%）。直径也显示出相似的趋势（高7%，p = 5.7%）。此外，老年组的毛细血管红细胞压积和密度显著较低（p < 5%），分别为32%和20%。(C)2014爱思唯尔公司All rights reserved.
Age-related decreases in baseline cerebral blood flow have been measured with various imaging modalities, however, the contribution of capillary flow to this phenomenon remain to elucidate. This study used 2-photon laser scanning fluorescence microscopy to measure capillary diameter, red blood cell speed, and flux in individual capillaries in the sensory-motor cortex of 12 adult (3-month-old) and 12 old (24-month-old) male Long-Evans rats under isoflurane anesthesia. The average (+/- standard deviation) diameter and speed over 921 capillaries were 6.4 +/- 1.4 mu m and 1.3 +/- 1.1 mm/s, respectively. Red blood cell speed and flux were significantly higher, by 48% and 15%, respectively, in old compared with young animals (p < 5%). The diameter also showed a similar tendency (7% higher, p = 5.7%). Furthermore, capillary hematocrit and density were significantly lower in the older group (p < 5%), by 32% and 20%, respectively. (C) 2014 Elsevier Inc. All rights reserved.