Terrestrial microorganisms at an altitude of 20,000 m in Earth's atmosphere
Terrestrial microorganisms at an altitude of 20,000 m in Earth's atmosphere
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DOI：
10.1023/b:aero.0000032948.84077.12
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发表时间：
2004-06-01
期刊：
影响因子：
2
通讯作者：
Griffin, Dale W.
中科院分区：
文献类型：
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作者：
Griffin, Dale W.
美国地质调查局 (USGS) 全球沙漠尘埃项目与 NASA 平流层和宇宙尘埃项目共同努力，从海拔 20,000 米采集的空气样本中鉴定出了可培养的微生物。分别使用形态学关键字和 16S rDNA 测序对总共 4 个真菌（青霉菌）和 71 个细菌菌落形成单位（70 个荧光芽孢杆菌菌落据信源自在高海拔地区收集的单个细胞和 1 个球形芽孢杆菌菌落）进行了计数、分离和鉴定。所有鉴定出的分离株都是陆地来源的产芽孢色素真菌或细菌，这表明地球高层大气中存在活微生物可能并不罕见。
A joint effort between the U.S. Geological Survey's (USGS) Global Desert Dust and NASA's Stratospheric and Cosmic Dust Programs identified culturable microbes from an air sample collected at an altitude of 20,000 m. A total of 4 fungal (Penicillium sp.) and 71 bacteria colony-forming units (70 colonies of Bacillus luciferensis believed to have originated from a single cell collected at altitude and one colony of Bacillus sphaericus) were enumerated, isolated and identified using a morphological key and 16S rDNA sequencing respectively. All of the isolates identified were spore-forming pigmented fungi or bacteria of terrestrial origin and demonstrate that the presence of viable microorganisms in Earth's upper atmosphere may not be uncommon.