Comparison of human adult and fetal expression and identification of 535 housekeeping/maintenance genes
Comparison of human adult and fetal expression and identification of 535 housekeeping/maintenance genes
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DOI：
10.1152/physiolgenomics.2000.2.3.143
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发表时间：
2000-04-27
影响因子：
4.6
通讯作者：
Tsyganskaya, M
中科院分区：
文献类型：
--
作者：
Warrington, JA;Nair, A;Tsyganskaya, M
使用高密度寡核苷酸阵列测量了 11 种不同成人和胎儿组织中约 7,000 个基因的基因表达水平，以鉴定参与细胞维持的基因。这些组织共享一组 535 个转录本，这些转录本在胎儿发育早期就被开启，并在整个成年期持续存在。因为我们的目标是识别正常个体中参与维持细胞功能的基因，所以我们通过筛选来自许多个体的 mRNA 来最大限度地减少个体变异的影响。该信息对于确定正常个体的平均表达水平很有用。此外，我们还鉴定了在 11 种组织中每种组织中独特表达的转录本。
Gene expression levels of about 7,000 genes were measured in 11 different human adult and fetal tissues using high-density oligonucleotide arrays to identify genes involved in cellular maintenance. The tissues share a set of 535 transcripts that are turned on early in fetal development and stay on throughout adulthood. Because our goal was to identify genes that are involved in maintaining cellular function in normal individuals, we minimized the effect of individual variation by screening mRNA pooled from many individuals. This information is useful for establishing average expression levels in normal individuals. Additionally, we identified transcripts uniquely expressed in each of the 11 tissues.