LIPOFECTION - A HIGHLY EFFICIENT, LIPID-MEDIATED DNA-TRANSFECTION PROCEDURE
LIPOFECTION - A HIGHLY EFFICIENT, LIPID-MEDIATED DNA-TRANSFECTION PROCEDURE
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DOI：
10.1073/pnas.84.21.7413
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发表时间：
1987-11-01
影响因子：
11.1
通讯作者：
DANIELSEN, M
中科院分区：
文献类型：
--
作者：
FELGNER, PL;GADEK, TR;DANIELSEN, M
已经开发了利用合成阳离子脂质N-[1-（2，3-二油基氧基）丙基]-N，N，N-三甲基氯化铵（DOTMA）的DNA转染方案。含有DOTMA的小单层脂质体自发地与DNA相互作用以形成脂质-DNA复合物，其中DNA被1005包埋。DOTMA促进复合物与组织培养细胞质膜的融合，导致DNA的摄取和表达。该技术简单，重复性好，对转染DNA的瞬时和稳定表达都有效。根据细胞系的不同，脂质体转染比磷酸钙或DEAE-葡聚糖转染技术有效5至> 100倍。
A DNA-transfection protocol has been developed that makes use of a synthetic cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA). Small unilamellar liposomes containing DOTMA interact spontaneously with DNA to form lipid-DNA complexes with 1005 entrapment of the DNA. DOTMA facilitates fusion of the complex with the plasma membrane of tissue culture cells, resulting in both uptake and expression of the DNA. The technique is simple, highly reproducible, and effective for both transient and stable expression of transfected DNA. Depending upon the cell line, lipofection is from 5- to > 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.