Modulation of CD4 by antigenic activation.
Modulation of CD4 by antigenic activation.
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DOI：
10.4049/jimmunol.138.5.1351
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发表时间：
1987-03
影响因子：
4.4
通讯作者：
C. Weyand;J. Goronzy;C. Fathman
中科院分区：
文献类型：
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作者：
C. Weyand;J. Goronzy;C. Fathman
CD 4是一种不变的膜糖蛋白，其细胞表面表达是MHC II类限制性T细胞辅助/诱导亚群的特征。虽然T淋巴细胞的特异性和限制性模式是由T细胞抗原受体决定的，但CD 4可能代表了一种额外的“相互作用”分子，其需要加强T细胞、抗原和抗原呈递细胞之间的相互作用。在这份手稿中，我们已经表明，CD 4的细胞表面表达与T细胞的活化相关。本文提供的数据首次证明，人T细胞克隆的抗原刺激导致CD 4标记物（以及CD 3标记物）的表达降低至组成型水平的约50%。如前所述，PMA引起CD 4和CD 3的调节，这表明蛋白激酶C的磷酸化在调节这两种标志物的表达中起着至关重要的作用。抗原刺激后CD 3和CD 4的平行下调表明，这两种标记物可能是介导T细胞活化的多分子复合物的成员。
Cell surface expression of CD4, an invariant membrane glycoprotein, is characteristic of the MHC class II-restricted T cell helper/inducer subset. Although the specificity and restriction patterns of T lymphocytes are determined by the T cell receptor for antigen, CD4 might represent an additional "interaction" molecule that is required to strengthen the interaction between T cells, antigen, and antigen-presenting cells. In this manuscript, we have shown that the cell surface expression of CD4 is correlated with activation of T cells. Data presented in this paper have demonstrated, for the first time, that antigenic stimulation of human T cell clones caused a decrease in the expression of the CD4 marker (as well as to the CD3 marker) to about 50% of the constitutive level. As previously demonstrated, PMA caused modulation of CD4 and CD3, which suggested that phosphorylation by protein kinase C played a crucial role in the regulation of the expression of both markers. The parallel down-regulation of CD3 and CD4 after antigen stimulation suggested that both markers might be members of a multimolecular complex mediating T cell activation.