VX2-induced rabbit buccal carcinoma: A potential cancer model for human buccal mucosa squamous cell carcinoma
VX2-induced rabbit buccal carcinoma: A potential cancer model for human buccal mucosa squamous cell carcinoma
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DOI：
10.1016/j.oraloncology.2009.07.012
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发表时间：
2009-11-01
期刊：
影响因子：
4.8
通讯作者：
Wang, Wen-Chen
中科院分区：
文献类型：
--
作者：
Lin, Li-Min;Chen, Yuk-Kwan;Wang, Wen-Chen
口腔粘膜是接触高剂量致癌物的习惯性嚼槟榔者患恶性肿瘤的最高风险部位。在所有的口腔鳞状细胞癌（SCC）中，颊粘膜的SCC与最差的病变最相关。因此，动物模型将有助于评估颊部SCC的新治疗方式。我们评估了VX 2兔癌模型是否可以用作人颊鳞癌的癌模型。10只成年雄性新西兰白色杂种兔随机分为A组（n = 2）和B组（n = 8）。将含有约40 × 10（6）个活细胞的0.5 ml VX 2肿瘤细胞悬液肌内注射到A组两只家兔的右后爪中。4周后，A组两只家兔的后爪SCC均为中分化至低分化。在两只动物中均未发现腹部器官转移，但发现多发性肺转移。随后将从A组动物获得的新鲜实体瘤块（约5 x 5 mm）插入B组8只家兔左颊的手术创建空间中。6周后，在所有8只动物中发现溃疡性颊部肿瘤（中度至低分化SCC）。在8只家兔中均未观察到内脏转移，但在8只动物中的4只中共发现11个平均2.75个颈部淋巴结转移。在一只动物中还观察到癌细胞侵入下颌骨和牙髓。总之，我们的研究结果表明，VX 2诱导的兔颊癌可能是一个潜在的癌症模型，为人类颊粘膜鳞状细胞癌。(C)2009爱思唯尔有限公司版权所有。
The buccal mucosa is the site at highest risk of contracting malignancy in habitual betel-quid chewers who expose the buccal mucosa to high doses of carcinogens. Of all oral squamous cell carcinomas (SCCs), those of the buccal mucosa are most associated with the poorest prognoses. Therefore, an animal model would be helpful to evaluate new treatment modalities for buccal SCC. We evaluated whether the VX2 rabbit cancer model could be employed as a cancer model for human buccal SCC. Ten adult male, New Zealand White outbred rabbits were randomly divided into two groups A (n = 2) and B (n = 8). A 0.5 ml VX2 tumor cell suspension containing approximately 40 x 10(6) vital cells was injected intramuscularly into the right hind paw of the two rabbits of group A. Four weeks later, moderately to poorly differentiated hind paw SCCs were apparent in both rabbits of group A. No abdominal organ metastases, but multiple pulmonary metastases, were found in both animals. Fresh solid tumor pieces (about 5 x 5 mm) obtained from group A animals were subsequently inserted into the surgically created spaces of the left cheeks of the eight rabbits of group B. Ulcerated buccal tumors (moderately to poorly differentiated SCCs) were found in all eight animals 6 weeks later. No internal organ metastases were noted in any of the eight rabbits, but a total of 11 with an average of 2.75 cervical lymph node metastases were found in four of the eight animals. Mandibular bone and tooth pulp invasion by cancer cells was also noted in one animal. In conclusion, our findings indicated that VX2-induced rabbit buccal carcinomas could be a potential cancer model for human buccal mucosa squamous cell carcinoma. (C) 2009 Elsevier Ltd. All rights reserved.