Optimization of Elicitation Conditions with Methyl Jasmonate and Salicylic Acid to Improve the Productivity of Withanolides in the Adventitious Root Culture of Withania somnifera (L.) Dunal
Optimization of Elicitation Conditions with Methyl Jasmonate and Salicylic Acid to Improve the Productivity of Withanolides in the Adventitious Root Culture of Withania somnifera (L.) Dunal
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DOI：
10.1007/s12010-012-9809-2
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发表时间：
2012-10-01
影响因子：
3
通讯作者：
Ganapathi, Andy
中科院分区：
文献类型：
--
作者：
Sivanandhan, Ganeshan;Arun, Muthukrishnan;Ganapathi, Andy
以睡茄（Withania somnifera（L.）杜纳尔处理茉莉酸甲酯和水杨酸独立。生物量积累，培养年龄，诱导期，培养持续时间进行了优化，以提高在两个最佳响应品种从Kolli山（东高止山脉）和坎本（西高止山脉）的泰米尔纳德邦，印度收集的睡茄内酯生产。在两种诱导剂中，水杨酸（SA）提高了科利山品种中主要睡茄内酯（睡茄内酯A、睡茄内酯B、睡茄素A和睡茄酮）以及次要成分（12-脱氧睡茄内酯、睡茄苷V和睡茄苷IV）的产量。根系生物量处理在30日龄的不定根培养物中，用11.70 g FW处理4 h，诱导子A、诱导子B、诱导子A分别产生64.65 mg g（-1）干重（DW）、33.74 mg g（-1）DW、17.47 mg g（-1）DW和20 mg g（-1）DW。与未处理的培养物相比，诱导10天后（培养第40天），42.88 mg g（-1）DW withanone（37倍）、5.34 mg g（-1）DW 12-脱氧withanoside monolide（9倍）、7.23 mg g（-1）DW withanoside V（7倍）和9.45 mg g（-1）DW withanoside IV（9倍）。这是首次报道利用诱导策略显著提高紫穗槐不定根培养中睡茄内酯产量。嗜睡。
Adventitious root cultures derived from leaf derived callus of Withania somnifera (L.) Dunal were treated with methyl jasmonate and salicylic acid independently. Biomass accumulation, culture age, elicitation period, and culture duration were optimized for higher withanolides production in the two best-responding varieties collected from Kolli hills (Eastern Ghats) and Cumbum (Western Ghats) of Tamil Nadu, India. Between the two elicitors, salicylic acid (SA) improved the production of major withanolides (withanolide A, withanolide B, withaferin A, and withanone) as well as minor constituents (12-deoxy withastramonolide, withanoside V, and withanoside IV) in the Kolli hills variety. Treatment of root biomass (11.70 g FW) on 30-day-old adventitious root cultures with 150 mu M SA for 4 h elicitor exposure period resulted in the production of 64.65 mg g(-l) dry weight (DW) withanolide A (48-fold), 33.74 mg g(-l) DW withanolide B (29-fold), 17.47 mg g(-l) DW withaferin A (20-fold), 42.88 mg g(-l) DW withanone (37-fold), 5.34 mg g(-l) DW 12-deoxy withastramonolide (nine fold), 7.23 mg g(-l) DW withanoside V (seven fold), and 9.45 mg g(-l) DW withanoside IV (nine fold) after 10 days of elicitation (40th day of culture) when compared to untreated cultures. This is the first report on the use of elicitation strategy on the significant improvement in withanolides production in the adventitious root cultures of W. somnifera.