The short N-terminus is required for functional expression of the virus-encoded miniature K+ channel Kcv
The short N-terminus is required for functional expression of the virus-encoded miniature K+ channel Kcv
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DOI：
10.1016/s0014-5793(02)03397-5
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发表时间：
2002-10-23
期刊：
影响因子：
3.5
通讯作者：
Thiel, G
中科院分区：
文献类型：
--
作者：
Moroni, A;Viscomi, C;Thiel, G
Kcv（K+ Chlorella virus）是一种小型病毒编码的K+通道。其预测的膜-孔-膜结构缺乏胞质C-末端，并且其具有短的12个氨基酸（aa）胞质N-末端。Kcv在人HEK 293细胞中表达时形成功能性通道。14个N-末端氨基酸的缺失导致Kcv蛋白的亚细胞位置和表达水平没有明显差异。然而，截短的蛋白质在转染的HEK 293细胞或爪蟾卵母细胞中不诱导可测量的电流。我们的结论是，N-末端控制的KCV通道的功能特性，但不影响蛋白质的表达。(C)2002年由Elsevier Science B. V.代表欧洲生物化学学会联合会出版。
Kcv (K+ Chlorella virus) is a miniature virus-encoded K+ channel. Its predicted membrane-pore-membrane structure lacks a cytoplasmic C-terminus and it has a short 12 amino acid (aa) cytoplasmic N-terminus. Kcv forms a functional channel when expressed in human HEK 293 cells. Deletion of the 14 N-terminal aa results in no apparent differences in the subcellular location and expression level of the Kcv protein. However, the truncated protein does not induce a measurable current in transfected HEK 293 cells or Xenopus oocytes. We conclude that the N-terminus controls functional properties of the Kcv channel, but does not influence protein expression. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.