Multiplex species-specific PCR identification of native giant clams in the South China Sea: A useful tool for application in giant clam stock management and forensic identification
Multiplex species-specific PCR identification of native giant clams in the South China Sea: A useful tool for application in giant clam stock management and forensic identification
复制标题
南海本地巨蛤的多重物种特异性 PCR 鉴定：用于巨蛤种群管理和法医鉴定的有用工具
DOI：
10.1016/j.aquaculture.2020.735991
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发表时间：
2020
期刊：
影响因子：
4.5
通讯作者：
Ziniu Yu
中科院分区：
文献类型：
--
作者：
Haitao Ma;Hongmei Gao;Yang Zhang;Yanping Qin;Zhiming Xiang;Jun Li;Yuehuan Zhang;Ziniu Yu
为了开发巨蛤物种鉴定的分子工具，本研究揭示了多重物种特异性PCR对中国南海五种巨蛤（Hippopus Hippopus,Tridacna maxima,Tridacna derasa,Tridacna squamosaandTridacna noae）的可靠、快速和低成本鉴定的有效性。我们使用线粒体COI基因进行标记开发。设计了5个反向引物和1个共同的正向引物，生成5个不同大小的种特异性PCR片段。这6个引物在多重PCR中组合，在一个反应中可以鉴定出5个巨型蛤蜊物种。该方法为南海巨蛤物种的简单、快速鉴定提供了一种新的方法，通过降低错误鉴定的风险，提高了巨蛤种群管理、保护和法医鉴定的效率和质量。
In an effort to develop molecular tools for giant clam species identification, this study reveals the usefulness of a multiplex species-specific PCR for the reliable, rapid and low-cost identification of five giant clam species found in the South China Sea:Hippopus hippopus, Tridacna maxima,Tridacna derasa,Tridacna squamosaandTridacna noae. We used the mitochondrial COI gene for marker development. Five reverse primers and a common forward primer were designed to generate five different size species-specific PCR fragments. These six primers were combined in a multiplex PCR to enable identification of five giant clam species in a single reaction. This molecular approach provides a simple and rapid identification of the giant clam species from the South China Sea, thus increasing the efficiency and quality of giant clam stock management, protecton and forensic identification by reducing the risk of wrong species identification.