A luteinizing hormone-releasing hormone agonist decreases biological activity and modifies chromatographic behavior of luteinizing hormone in man.
A luteinizing hormone-releasing hormone agonist decreases biological activity and modifies chromatographic behavior of luteinizing hormone in man.
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黄体生成素释放激素激动剂降低人体黄体生成素的生物活性并改变其色谱行为。
DOI：
10.1172/jci111200
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Rabin,D
中科院分区：
文献类型：
--
作者：
Evans,RM;Doelle,GC;Lindner,J;Bradley,V;Rabin,D
促黄体生成激素释放激素（LHRH）激动剂，[D-Trp 6，Pro 9-NEth]LHRH（LHRHA），对促黄体生成激素（LH）生物活性的影响进行了评估，在14天的治疗期间，大鼠间质细胞测定。生物学/免疫学（B/I）比值最初在给药后未发生变化，但到第12天已降至低于基础值的水平。第12天频繁采样显示促性腺激素对LHRHA的反应迟钝，无自发性LH脉动。尽管继续给予LHRHA，但人绒毛膜促性腺激素给药导致B/I比值和睾酮水平升高。通过葡聚糖凝胶色谱法对血清免疫反应性LH的进一步表征显示，在用LHRHA治疗期间，洗脱曲线较晚。因此，LHRHA似乎部分通过改变LH在人体中的生物活性而起作用。
The effect of the luteinizing hormone-releasing hormone (LHRH) agonist, [D-Trp6,Pro9-NEth]LHRH (LHRHA), on luteinizing hormone (LH) bioactivity was assessed with a rat interstitial cell assay in four men during a 14-d treatment period. Biologic/immunologic (B/I) ratios were unchanged initially with treatment but by day 12 had fallen to levels lower than basal values. Frequent sampling on day 12 revealed blunted gonadotropin responsiveness to LHRHA and absence of spontaneous LH pulsations. Despite continued administration of LHRHA, human chorionic gonadotropin administration resulted in elevated B/I ratios and testosterone levels. Further characterization of the serum immunoreactive LH by Sephadex chromatography revealed a later elution profile during treatment with LHRHA. Thus, LHRHA appears to act, in part, by modification of the bioactivity of LH in man.