Human U937 monocyte behavior and protein expression on various formulations of three-dimensional fibrin clots
Human U937 monocyte behavior and protein expression on various formulations of three-dimensional fibrin clots
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DOI：
10.1111/j.1524-475x.2005.00091.x
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发表时间：
2006-01-01
影响因子：
2.9
通讯作者：
Tawil, B
中科院分区：
文献类型：
--
作者：
Mana, M;Cole, M;Tawil, B
诸如Tisseel（R）（巴克斯特医疗保健公司）的柔性密封剂产品用于止血和组织密封。Tisseel（R）由两种成分组成，一种含纤维蛋白原的成分和凝血酶，当它们混合在一起时形成纤维蛋白凝块。人们对将单核细胞递送至伤口感兴趣，因为已知它们在伤口愈合过程中发挥重要作用。因此，我们感兴趣的是通过检查单核细胞在3D-纤维蛋白凝块上的行为来找到用于递送单核细胞的最佳纤维蛋白制剂。使用标准的粘附和增殖试验，我们发现单核细胞差异粘附，增殖，并根据最终的纤维蛋白原和凝血酶浓度的3D-纤维蛋白凝块上和内集群。此外，使用Boyden室测定，我们发现单核细胞在1-2天内迁移通过3D-纤维蛋白凝块。此外，在不同时间点接种在3D-纤维蛋白凝块上的单核细胞中的蛋白质表达根据最终3D-纤维蛋白凝块中的纤维蛋白原和凝血酶浓度而变化。上述数据表明，纤维蛋白封闭剂Tisseel（R）的各种制剂在体外和体内伤口愈合过程中为单核细胞粘附、增殖和迁移提供了良好的表面。此外，由于单核细胞增殖良好并聚集在3D纤维蛋白中，因此Tisseel（R）可能是将单核细胞递送到慢性伤口中以克服愈合缺陷的良好递送载体。
Fibrin sealant products such as Tisseel(R) (Baxter Healthcare Corporation) are used in hemostasis and tissue sealing. Tisseel(R) consists of two components, a fibrinogen-containing component and thrombin, which when mixed together form a fibrin clot. There is an interest in delivering monocytes to the wound because they are known to play an important role in the wound-healing process. Therefore, we were interested in finding the best fibrin formulation for delivering monocytes by examining monocyte behavior on 3D-fibrin clots. Using standard adhesion and proliferation assays, we found that monocytes differentially adhere, proliferate, and cluster on and within the 3D-fibrin clots depending on the final fibrinogen and thrombin concentration. Moreover, using a Boyden chamber assay, we found that monocytes migrated through the 3D-fibrin clots in 1-2 days. Furthermore, the protein expression in monocytes seeded on 3D-fibrin clots for various time points varied depending on the fibrinogen and thrombin concentrations in the final 3D-fibrin clots. The above data suggest that various formulations of fibrin sealant Tisseel(R) present a good surface for monocyte adhesion, proliferation and migration in vitro and potentially during the wound-healing process in vivo. Moreover, because the monocytes proliferated well and clustered in the 3D fibrin, Tisseel(R) could be a good delivery vehicle for delivering monocytes into chronic wounds to overcome a healing deficiency.