Role of HIV-1 nucleocapsid protein in HIV-1 reverse transcription
Role of HIV-1 nucleocapsid protein in HIV-1 reverse transcription
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DOI：
10.4161/rna.7.6.14115
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发表时间：
2010-11-01
期刊：
影响因子：
4.1
通讯作者：
Musier-Forsyth, Karin
中科院分区：
文献类型：
--
作者：
Levin, Judith G.;Mitra, Mithun;Musier-Forsyth, Karin
HIV-1核衣壳蛋白（NC）是一种核酸分子伴侣，它可以重塑核酸结构，从而形成最稳定的构象。这种活性对于病毒复制是必不可少的，并且在介导高度特异性和有效的逆转录中具有关键作用。NC在该过程中的功能取决于三种性质：（1）聚集核酸的能力;（2）适度的双链体去稳定化活性;和（3）快速的开-关结合动力学。在这里，我们提出了一个详细的分子分析过程中发生的病毒DNA合成的个别事件，并显示如何NC的属性是重要的，几乎每一个步骤的途径。最后，我们还回顾了感染期间逆转录的生物学方面以及NC、逆转录酶和人APOBEC 3G之间的相互作用，APOBEC 3G是一种HIV-1限制因子，在不存在HIV-1 Vif蛋白的情况下抑制逆转录和病毒复制。
The HIV-1 nucleocapsid protein (NC) is a nucleic acid chaperone, which remodels nucleic acid structures so that the most thermodynamically stable conformations are formed. This activity is essential for virus replication and has a critical role in mediating highly specific and efficient reverse transcription. NC's function in this process depends upon three properties: (1) ability to aggregate nucleic acids; (2) moderate duplex destabilization activity; and (3) rapid on-off binding kinetics. Here, we present a detailed molecular analysis of the individual events that occur during viral DNA synthesis and show how NC's properties are important for almost every step in the pathway. Finally, we also review biological aspects of reverse transcription during infection and the interplay between NC, reverse transcriptase and human APOBEC3G, an HIV-1 restriction factor that inhibits reverse transcription and virus replication in the absence of the HIV-1 Vif protein.