Transcriptional and post-transcriptional control of specific messenger RNAs in adult and embryonic liver.
Transcriptional and post-transcriptional control of specific messenger RNAs in adult and embryonic liver.
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成人和胚胎肝脏中特定信使 RNA 的转录和转录后控制。
DOI：
10.1016/0022-2836(84)90304-8
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发表时间：
1984
影响因子：
5.6
通讯作者：
DarnellJr,JE
中科院分区：
文献类型：
--
作者：
Powell,DJ;Friedman,JM;Oulette,AJ;Krauter,KS;DarnellJr,JE
合成速率和各种信使RNA序列的浓度之间进行了比较成年小鼠肝细胞和其他成年组织中的细胞，以及胎儿和新生儿肝脏中的细胞。在以前的报告中，这些序列被区分为“肝脏特异性”或“常见”，其中肝脏与脑细胞和培养细胞进行了比较。大多数肝脏特异性mRNA在大量其他成年小鼠组织中大大减少或缺失。在两种情况下，肾脏与肝脏共享mRNA序列。在不同组织中，常见mRNA的水平从2倍到5倍不等。对于肝脏特异性mRNA，成人组织和胎肝细胞核中的转录率与mRNA的存在和水平表现出良好的对应关系。然而，大多数常见的RNA序列在所有细胞核中以相似的速率转录，尽管它们的细胞质浓度不同。此外，两个肝脏特异性RNA在胎肝细胞核中转录，但不作为mRNA存在。因此，组织特异性mRNA在成体细胞中的存在首先基于（并且可能是最重要的）转录控制，但是观察到转录后控制也有助于mRNA水平的几个实例。
The rate of synthesis and the concentrations of a variety of messenger RNA sequences have been compared between adult mouse liver cells and cells in other adult tissues, and between cells in fetal and neonatal liver. The sequences were distinguished as “liver-specific” or “common” in a previous report, where liver was compared with brain cells and cultured cells. Most of the liver-specific mRNAs are greatly decreased or absent in a large group of other adult mouse tissues. In two cases, kidney shares mRNA sequences with liver. The levels of the common mRNAs varied from two to fivefold in various tissues. For the liver-specific mRNAs, the transcription rates in nuclei from adult tissues and from fetal liver showed a good correspondence to the presence and the level of mRNA. However, most of the common RNA sequences were transcribed at similar rates in all nuclei despite their different cytoplasmic concentrations. In addition, two liver-specific RNAs were transcribed in fetal liver nuclei but were not present as mRNA. Thus, the presence of tissue-specific mRNAs in adult cells is based first (and probably most importantly) on transcriptional control, but several instances were observed where post-transcriptional control also contributes to the level of mRNA.