Effect of endorphins on amylase secretion from rat pancreasIn vivo andIn vitro
Effect of endorphins on amylase secretion from rat pancreasIn vivo andIn vitro
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DOI：
10.1007/bf01347894
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发表时间：
1985-03
影响因子：
3.1
通讯作者：
Manjit Singh
中科院分区：
文献类型：
--
作者：
Manjit Singh
本研究采用大鼠胰管插管活体动物模型，在离体胰腺切片和游离腺泡中观察内源性阿片肽对胰腺腺泡细胞的作用方式。胰腺切片与β-内啡肽或(蛋氨酸)-5-脑啡肽单独孵育及与CCK8联合孵育。分离的腺泡分别与纳洛酮、P物质、血管活性肠肽、(Met)5-和(Leu)5-脑啡肽和α-，β-以及γ-内啡肽单独或与CCK8共同孵育。在体内，β-内啡肽和(Met)5-脑啡肽均不改变基础分泌，但抑制CCK8刺激的淀粉酶分泌。这种作用不能被纳洛酮逆转。在脑片中，β-内啡肽和(Met)-5-脑啡肽都不改变基础分泌或CCK8刺激的分泌。在分离的腺泡中，P物质和VIP显著增加淀粉酶的分泌，而纳洛酮、脑啡肽和内啡肽不能改变淀粉酶的分泌。CCK8使淀粉酶分泌量增加6倍以上。脑啡肽和内啡肽对CCK8效应既无抑制作用，也无增强作用。这些数据表明，阿片肽对大鼠胰腺腺泡细胞的作用是非特异性的，似乎不是由阿片受体介导的。
The present study was done bothin vivoby cannulating pancreatic duct of rats andin vitrousing pancreatic slices and dissociated acini to determine the mode of action of endogenous opiate peptides on pancreatic acinar cell. Pancreatic slices were incubated with β-endorphin or (Met)5-enkephalin alone and in combination with CCK8. Dissociated acini were incubated with naloxone, substance P, VIP, (Met)5-and (Leu)5-enkephalin and α-, β-, and γ-endorphin alone or in combination with CCK8.In vivo, both β-endorphin and (Met)5-enkephalin did not alter basal secretion but inhibited CCK8-stimulated amylase secretion. This effect was not reversed by administration of naloxone. In the slices, neither β-endorphin nor (Met)5-enkephalin altered basal or CCK8-stimulated secretion. In the dissociated acini, substance P and VIP significantly increased amylase secretion, whereas naloxone, enkephalins, and endorphins failed to alter amylase secretion. CCK8increased amylase secretion greater than sixfold. In combination with enkephalins and endorphins, there was neither inhibition nor potentiation of CCK8effect. These data indicate that the effect of opiate peptides on pancreatic acinar cells in the rat are nonspecific and appear not to be mediated by opiate receptors.