Monitoring renal cryosurgery: Predictors of tissue necrosis in swine
Monitoring renal cryosurgery: Predictors of tissue necrosis in swine
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DOI：
10.1016/s0022-5347(01)63618-8
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发表时间：
1998-04-01
影响因子：
6.6
通讯作者：
Warner, TF
中科院分区：
文献类型：
--
作者：
Chosy, SG;Nakada, SY;Warner, TF
目的：在猪模型中检查用于监测肾冷冻手术的参数并与组织病理学坏死相关。参数包括：1）组织温度，2）组织与冷冻外科探针插入部位的距离，以及3）可见冰球对组织的包裹。材料和方法：中线切开后，使用氮基冷冻外科系统治疗3头家猪的6个健康肾脏，冷冻外科系统具有3.4 mm外径冷冻探针。使用原型热电偶在每个肾脏的6个部位监测温度。大体和组织学分析进行收获从每个热敏站点postoperation.Results 24小时：所有动物存活完成完整的协议。手术治疗术后出血。组织学显示，在-19.4 ℃或更低的温度下，13/13的组织样本均匀地产生了完全消融。在探针插入部位的16 mm范围内，17/17个样本中的细胞被均匀消融，而超出该范围的组织死亡程度变化很大。在可见的冰球所包围的点（16/18，89%）比在其之外的点（2/18，11%）更可能发现细胞死亡[p
Purpose: To examine parameters for monitoring renal cryosurgery and correlate with histopathologic necrosis in a porcine model. Parameters include: 1) tissue temperature, 2) distance of tissue from the cryosurgical probe insertion site, and 3) inclusion of tissue by the visible iceball.Materials and Methods: Following midline incision, 6 healthy kidneys from 3 domestic pigs were treated using a nitrogen-based cryosurgical system with 3.4 mm outer diameter cryoprobe. Temperatures were monitored at 6 sites in each kidney using prototype thermocouples. Gross and histologic analysis was performed on tissue harvested from each thermosensor site 24 hours postoperatively.Results: All animals survived to complete the full protocol. Post-procedure bleeding was managed surgically. Histopathology revealed that complete ablation was uniformly produced at temperatures of -19.4C or lower in 13/13 tissue samples. Within 16 mm. of the probe insertion site, cells were uniformly ablated in 17/17 samples while degree of tissue death varied widely beyond this margin. Cell death was more likely found at points encompassed by the visible iceball (16/18, 89%) than those beyond it (2/18, 11%) [p