Superoxide generation by the human polymorphonuclear leukocyte in response to latex beads.
Superoxide generation by the human polymorphonuclear leukocyte in response to latex beads.
复制标题
人类多形核白细胞对乳胶珠的反应产生超氧化物。
DOI：
10.1002/jlb.51.6.591
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发表时间：
1992
影响因子：
5.5
通讯作者：
Shirley,PS
中科院分区：
文献类型：
--
作者：
Thomas,MJ;Hedrick,CC;Smith,S;Pang,J;Jerome,WG;Willard,AS;Shirley,PS
在这项研究中，当多形核白细胞被亚油醇包裹的、羧基修饰的乳胶珠处理时，没有立即检测到超氧化物的形成。然而，所有其他中性粒细胞激活的指标都存在。直到首次接触珠子后30分钟才检测到超氧化物。然而，一种产生O-−-2的、依赖于NADPH2的氧化酶在暴露15分钟后仍处于活性状态。当用细胞松弛素B处理多形核白细胞时，在接触珠子后立即检测到超氧化物的产生。用涂有亚油醇的乳胶珠治疗后的超氧化物分泌与其他乳胶珠的反应进行了比较。结果表明，中性粒细胞在涂有亚油醇的乳胶珠周围形成一个吞噬酶体，紧密密封，不允许超氧化物逃逸到介质中，在那里可以通过还原铁细胞色素c来检测到它。
In this study, superoxide formation was not immediately detected when polymorphonuclear leukocytes were treated with linoleyl alcohol–coated, carboxy‐modified latex beads. However, all other measures of neutrophil activation were present. Superoxide was not detected until 30 min after the initial exposure to beads. However, an O−2‐producing, NADPH‐dependent oxidase is active 15 min after exposure. When polymorphonuclear leukocytes are pretreated with cytochalasin B, superoxide production is detected immediately after exposure to the beads. Superoxide secretion after treatment with linoleyl alcohol–coated latex beads is compared with the response to other latex beads. The results imply that neutrophils form a phagolysosome around linoleyl alcohol–coated latex beads that is tightly sealed and does not allow superoxide to escape into the medium where it could be detected by the reduction of ferricytochrome c.