Recombinant interferon-gamma stimulates the production of human tumor necrosis factor in vitro.
Recombinant interferon-gamma stimulates the production of human tumor necrosis factor in vitro.
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发表时间：
1987
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通讯作者：
P. Scuderi;K. Sterling;A. Raitano;T. Grogan;R. Rippe
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作者：
P. Scuderi;K. Sterling;A. Raitano;T. Grogan;R. Rippe
培养的人外周血单核细胞分泌肿瘤坏死因子（TNF），可以用灵敏的酶联免疫吸附测定系统检测。当将重组人干扰素-γ（rIFN-γ）添加到培养的细胞中时，TNF产生增加。rIFN-γ还能够在4天的时间内维持TNF的升高的产生水平。重组干扰素-α（rIFN-α）不能刺激TNF的产生增加。未经刺激的单核吞噬细胞和rIFN-α处理的细胞在4天的时间间隔内减少TNF的分泌。rIFN-γ的刺激作用是剂量依赖性的，需要新的RNA和蛋白质的合成，并且不依赖于组织培养基中的内毒素。
Human peripheral blood monocytes in culture secrete tumor necrosis factor (TNF), which can be detected with a sensitive enzyme-linked immunosorbent assay system. When recombinant human interferon-gamma (rIFN-gamma) is added to cultured cells, TNF production is increased. rIFN-gamma is also able to sustain the elevated production level of TNF over a 4-day period. Recombinant interferon-alpha (rIFN-alpha) was unable to stimulate increased TNF production. Unstimulated mononuclear phagocytes and rIFN-alpha-treated cells decreased secretion of TNF over a 4-day interval. The stimulatory effect of rIFN-gamma was dose dependent and required both new RNA and protein synthesis and was independent of endotoxin in the tissue culture medium.