Bacteriophage Treatment Reduces Salmonella Colonization of Infected Chickens
Bacteriophage Treatment Reduces Salmonella Colonization of Infected Chickens
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DOI：
10.1637/8091-082007-reg
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发表时间：
2008-03-01
期刊：
影响因子：
1.4
通讯作者：
Robeson, J.
中科院分区：
文献类型：
--
作者：
Borie, C.;Albala, I.;Robeson, J.
从商业鸡群的污水系统中分离出三种不同的裂解噬菌体(BP)，并用于减少实验鸡的肠炎沙门氏菌(SE)定植。10日龄雏鸡以9.6×10(5)个菌落形成单位(CFU)/毫升的SE毒株攻击，在SE攻击前24小时，在10(3)个空斑形成单位(PFU)的多重感染(MOI)下用粗喷雾或含三种噬菌体的鸡尾酒处理。鸡在20日龄时被安乐死，进行个体SE检测、定量细菌学和从肠道和器官池中分离噬菌体。Se检测采用细菌学培养和基因组聚合酶链式反应(PCR)检测。定性细菌学结果显示，BPS气雾喷雾剂能显著降低鸡的SE感染率(P=0.0084)，与对照组(100%)相比，下降了72.7%。此外，SE计数显示，粗喷雾和饮水均能降低肠道SE的定殖率(P
Three different lytic bacteriophages ( BPs) were isolated from the sewage system of commercial chicken flocks and used to reduce Salmonella Enteritidis ( SE) colonization from experimental chickens. Ten-day-old chickens were challenged with 9.6 3 X 10(5) colony-forming units ( CFU)/ ml of a SE strain and treated by coarse spray or drinking water with a cocktail of the three phages at a multiplicity of infection ( MOI) of 10(3) plaque-forming units ( PFU) 24 hr prior to SE challenge. Chickens were euthanatized at day 20 of age for individual SE detection, quantitative bacteriology, and phage isolation from the intestine and from a pool of organs. SE detection was performed by both bacteriologic culture and genome detection by polymerase chain reaction ( PCR). Qualitative bacteriology showed that aerosol-spray delivery of BPs significantly reduced the incidence of SE infection in the chicken group ( P = 0.0084) to 72.7% as compared with the control group ( 100%). In addition, SE counts showed that phage delivery both by coarse spray and drinking water reduced the intestinal SE colonization ( P