Automation of urine sediment examination: A comparison of the sysmex UF-100 automated flow cytometer with routine manual diagnosis (microscopy, test strips, and bacterial culture)
Automation of urine sediment examination: A comparison of the sysmex UF-100 automated flow cytometer with routine manual diagnosis (microscopy, test strips, and bacterial culture)
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DOI：
10.1515/cclm.1999.116
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发表时间：
1999-07-01
影响因子：
6.8
通讯作者：
Kampf, SC
中科院分区：
文献类型：
--
作者：
Hannemann-Pohl, K;Kampf, SC
使用UF-100流式细胞仪（Sysmex TOA Medical Electronics（Europe）GmbH，Hamburg，德国）和手动显微镜和试纸检查438例患者的尿液标本。其中142例还进行了细菌学检查。使用UF-100对未预先离心的天然尿液进行测量。测定内不精密度，红细胞的CV为1.3%（547/μ l）至8.5%（24/μ l），白细胞的CV为2.4%（218/μ l）至5.6%（10/μ l），与临床化学中常见的结果相似，并且远优于沉淀物手动显微镜检查中观察到的结果。在常规使用中，在9%的标本中观察到颗粒浓度过高导致流式细胞仪过载。应目视检查此类标本。UF-100在测定白细胞、红细胞、上皮细胞和细菌方面明显比手动显微镜更灵敏。根据所得结果估计参考范围。这些使UF-100能够取代常规的人工诊断方法。虽然灵敏度比手工镜检提高了，但在测定红细胞以检测溶血时，总是需要进行平行试纸检查，我们认为Sysmex UF-100是手动镜检尿沉渣的合适替代品。此外，它提供了一个机会，以提高标准化的基本尿液分析。
Urine specimens from 438 patients were examined with the UF-100 flow cytometer (Sysmex TOA Medical Electronics (Europe) GmbH, Hamburg, Germany) and by manual microscopy and test strips. One hundred and forty-two of these were also examined bacteriologically. The measurements with the UF-100 were performed on native urine without prior centrifugation. Intraassay imprecision, CV of 1.3 % (547/mu l) to 8.5 % (24/mu l) for erythrocytes and CV of 2.4 % ( 218/mu l) to 5.6 % (10/mu l) for leukocytes, are similar to those usual in clinical chemistry, and are very much better than those seen in manual microscopy of sediment. In routine use, overloading the flow cytometer by an excessive concentration of particles was observed in 9 % of specimens. Such specimens should be checked visually. The UF-100 is distinctly more sensitive than manual microscopy for determining leukocytes, erythrocytes, epithelial cells and bacteria. Reference ranges were estimated from the results obtained. These enabled the UF-100 to replace routine manual diagnostic methods. Although the sensitivity is improved over manual microscopy of sediment, it is always necessary to perform parallel test strip examination when determining erythrocytes in order to detect haemolysis.In our opinion the Sysmex UF-100 is a suitable replacement for manual microscopy of urine sediment. In addition it offers an opportunity to improve standardization of basic urinalysis.