Whole-genome sequence analysis of paris virus 1: a novel member of the genus Potyvirus infecting Paris polyphylla var. yunnanensis
Whole-genome sequence analysis of paris virus 1: a novel member of the genus Potyvirus infecting Paris polyphylla var. yunnanensis
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巴黎病毒1号全基因组序列分析：感染重楼的马铃薯蒂病毒属新成员。
DOI：
10.1007/s00705-020-04560-3
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发表时间：
2020-02-25
影响因子：
2.7
通讯作者：
Zhao, Mingfu
中科院分区：
文献类型：
--
作者：
Chen, Lu;Anane, Rex Frimpong;Zhao, Mingfu
本文报道了一种感染多叶巴黎变种的新型马铃薯Y病毒（potyvirus）的全基因组序列，该病毒暂命名为“巴黎病毒1”（ParV 1，GenBank登录号MN 549985）。云南白药是云南白药中的一种。在ParV 1基因组中检测到一个编码3240-aa多聚蛋白的单个大开放阅读框（nt 96-9818），该多聚蛋白被预测切割成10个成熟蛋白。ParV 1基因组序列与其他马铃薯Y病毒的比较分析确定了9个切割位点和保守的基序，这是马铃薯Y病毒的典型特征。两两序列比较表明，ParV 1多聚蛋白与马铃薯Y病毒属病毒的核苷酸同源性为49.6-65.1%，氨基酸同源性为47.1-68.9%。BLAST分析表明，ParV 1与浙贝母花叶病毒（TFMV，accession no.CAI59123）有65.1%的核苷酸和68.9%的氨基酸序列同源性。这些结果表明，巴黎病毒1型（ParV 1）是马铃薯Y病毒属的一个新成员。
The complete genome sequence of a novel potyvirus, tentatively named "paris virus 1" (ParV1, GenBank accession no. MN549985), infecting Paris polyphylla var. yunnanensis was determined in this study. A single large open reading frame (nt 96-9818) encoding a 3240-aa polyprotein that is predicted to be cleaved into 10 mature proteins was detected in the ParV1 genome. Comparative analysis of the ParV1 genome sequence with those of other potyviruses identified nine cleavage sites and conserved motifs that are typical features of potyviruses. Pairwise sequence comparisons showed that the ParV1 polyprotein shares 49.6-65.1% nucleotide and 47.1-68.9% amino acid sequence identity with viruses of the genus Potyvirus. BLAST analysis revealed that ParV1 shares 65.1% nucleotide and 68.9% amino acid sequence identity with Thunberg fritillary mosaic virus (TFMV, accession no. CAI59123), its closest known relative. These results suggest that paris virus 1 (ParV1) is a new member of the genus Potyvirus.