Resveratrol Delivery from Implanted Cyclodextrin Polymers Provides Sustained Antioxidant Effect on Implanted Neural Probes
Resveratrol Delivery from Implanted Cyclodextrin Polymers Provides Sustained Antioxidant Effect on Implanted Neural Probes
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DOI：
10.3390/ijms21103579
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发表时间：
2020-05-01
影响因子：
5.6
通讯作者：
Ereifej, Evon S.
中科院分区：
文献类型：
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作者：
Haley, Rebecca M.;Zuckerman, Sean T.;Ereifej, Evon S.
皮质内微电极是研究和治疗神经系统疾病的重要工具。在很大程度上由于电极植入后发生的氧化应激和炎症反应，这些电极的信号质量随时间降低。为了减轻这种反应，白藜芦醇，一种天然的抗氧化剂，通过减少氧化应激增强神经保护作用，被利用。这项工作比较了传统的全身递送白藜芦醇的新的环糊精聚合物（pCD）的局部递送方法，在体外和体内。pCD显示延长的白藜芦醇释放100天，以及60天的自由基清除活性在体外。体内结果表明，我们的pCD递送系统成功地将白藜芦醇递送到大脑，在整个短期研究（长达7天）中持续释放。有趣的是，与全身施用白藜芦醇相比，在皮质内探针植入部位发现了显著更高浓度的白藜芦醇代谢物。总之，我们的试点结果提供了支持的可能性，改善白藜芦醇的交付，试图稳定长期的神经接口应用。
Intracortical microelectrodes are valuable tools used to study and treat neurological diseases. Due in large part to the oxidative stress and inflammatory response occurring after electrode implantation, the signal quality of these electrodes decreases over time. To alleviate this response, resveratrol, a natural antioxidant which elicits neuroprotective effects through reduction of oxidative stress, was utilized. This work compares traditional systemic delivery of resveratrol to the novel cyclodextrin polymer (pCD) local delivery approach presented herein, both in vitro and in vivo. The pCD displayed an extended resveratrol release for 100 days, as well as 60 days of free radical scavenging activity in vitro. In vivo results indicated that our pCD delivery system successfully delivered resveratrol to the brain with a sustained release for the entire short-duration study (up to 7 days). Interestingly, significantly greater concentrations of resveratrol metabolites were found at the intracortical probe implantation site compared to the systemic administration of resveratrol. Together, our pilot results provide support for the possibility of improving the delivery of resveratrol in an attempt to stabilize long-term neural interfacing applications.