Transcriptome analysis of tetrodotoxin sensing and action of tetrodotoxin in central nervous system of tiger puffer Takifugu rubripes juveniles
Transcriptome analysis of tetrodotoxin sensing and action of tetrodotoxin in central nervous system of tiger puffer Takifugu rubripes juveniles
复制标题
红鳍东方鲀幼鱼中枢神经系统河豚毒素传感和作用的转录组分析
DOI：
10.1007/s12562-017-1079-z
复制
发表时间：
2017
影响因子：
1.9
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Okita;K.;Tan;E.;Satone;H.;Kinoshita;S.;Asakawa;S.;Ojima;D.;Yamazaki;H.;Sakiyama;K.;Takatani;T.;Arakawa;O.;Hagiwara;A.;Sakakura;Y.
为了揭示河豚幼鱼对河豚毒素(TTX)的感知及其在中枢神经系统(CNS)中的作用，我们利用下一代测序技术对服用河豚毒素的无毒养殖幼鱼的嗅觉系统和脑进行了转录组学分析。6700万个来自鼻区(嗅觉上皮和皮肤)和大脑的读数分别来自对照组、河豚毒素敏感组和河豚毒素注射组的三个个体，被组装成153,958个重叠群。将每个样本的原始读数映射到Thet中预测转录本的核苷酸序列。对其表达频率的研究表明，21个已知基因和81个已知基因在TTX敏感和注射TTX的幼虫中的表达分别与对照幼虫相比发生了显著变化。这些基因包括与摄食调节和奖励系统相关的基因，表明TTX经常摄入。幼虫在大脑的摄食中心受到控制。红葡萄酒可能会感觉到河豚毒素是一种奖励，而累积的河豚毒素直接作用于中枢神经系统来调节河豚毒素的摄取。
To reveal the sensing of tetrodotoxin (TTX) by tiger pufferTakifugu rubripesjuveniles and its action in the central nervous system (CNS), we conducted transcriptome analysis using next-generation sequencing for the olfactory system and brain of non-toxic cultured juveniles administered TTX. Sixty-seven million reads from the nasal region (olfactory epithelium and skin) and the brain of each of three individuals of the control, TTX-sensing and TTX-administered juveniles were assembled into 153,958 contigs. Mapping raw reads from each sample onto the nucleotide sequences of predicted transcripts in theT. rubripesgenome (FUGU version 4) and the de novo assembled contigs to investigate their frequency of expression revealed that the expression of 21 and 81 known genes significantly changed in TTX-sensing and TTX-administered juveniles in comparison with control juveniles, respectively. These genes included those related to feeding regulation and a reward system, and indicated that TTX ingestion ofT. rubripesjuveniles is controlled in the feeding center in the brain, thatT. rubripesmay sense TTX as a reward, and that accumulated TTX directly acts on the central nervous system to adjust TTX ingestion.