Nickel allergy and relationship with Staphylococcus aureus in atopic dermatitis
Nickel allergy and relationship with Staphylococcus aureus in atopic dermatitis
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DOI：
10.1016/j.jtemb.2015.06.009
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发表时间：
2016-01-01
影响因子：
3.5
通讯作者：
Krystyna, Obtulowicz
中科院分区：
文献类型：
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作者：
Bogdali, Anna M.;Grazyna, Antoszczyk;Krystyna, Obtulowicz
背景资料：镍空气污染的增加被认为是镍作用的频繁副作用，与特应性皮炎中镍过敏患者的金黄色葡萄球菌的毒力潜力有关。目的是探讨镍过敏与链球菌感染的关系。方法：采用斑贴试验在特应性皮炎患者中确认镍过敏，并在健康志愿者中排除镍过敏。感染S.用API Staph系统检测过敏性患者和健康志愿者的金黄色葡萄球菌。检测金黄色葡萄球菌肠毒素A和B的特异性IgE。用ELISA pot法检测PBMC在硫酸镍和肠毒素A、B刺激下分泌IFN-g、IL-2、IL-13的情况。与无镍过敏的特应性患者和健康志愿者相比，镍过敏的特应性患者中的金黄色葡萄球菌。硫酸镍刺激下IL-2分泌的增加仅见于感染了链球菌的镍过敏症患者。结论：镍过敏和金黄色葡萄球菌感染与镍中毒有关。金黄色葡萄球菌与特应性皮炎有关(C)2015 Elsevier GmbH. All rights reserved.
Background: The increase of nickel air pollution is supposed to frequent side effects of nickel action related to virulence potential of Staphylococcus aureus in patients with nickel allergy in atopic dermatitis. The goal was to investigate the relationship between nickel allergy and infection by S. aureus in atopic dermatitis.Methods: Nickel allergy was confirmed in atopic patients and excluded in healthy volunteers using patch testing. Infection by S. aureus was tested in atopic patients and healthy volunteers by use of API Staph system. The specific IgE for staphylococcal enterotoxin A and B were measured. Secretion of IFN-g, IL-2, IL-13 by PBMC under nickel sulfate and the enterotoxins A and B stimulations were studied with ELISpot.Results: We found the increased number of infections by S. aureus in atopic patients with nickel allergy in comparison to atopic patients and healthy volunteers without nickel allergy. The elevated secretion of IL-2 under nickel sulfate stimulation in vitro was exclusively found in atopic patients with nickel allergy infected by S. aureus.Conclusions: Our data suggest that nickel allergy and infection by S. aureus are linked in atopic dermatitis. (C) 2015 Elsevier GmbH. All rights reserved.