Cyclin-like F-box protein plays a role in growth and development of the three model species Medicago truncatula, Lotus japonicus, and Arabidopsis thaliana
Cyclin-like F-box protein plays a role in growth and development of the three model species Medicago truncatula, Lotus japonicus, and Arabidopsis thaliana
复制标题
DOI：
10.2147/rrb.s84753
复制
发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Iantcheva, Anelia
中科院分区：
文献类型：
--
作者：
Boycheva, Irina;Vassileva, Valya;Iantcheva, Anelia
在真核生物中，F-box蛋白是SCF复合物的主要组成部分之一，属于泛素E3连接酶家族，催化蛋白质的泛素化，维持蛋白质合成与降解的平衡。本研究利用转基因模式植物蒺藜苜蓿，阐明了编码细胞周期蛋白样F-box蛋白基因的作用和功能。通过农杆菌介导的转化产生的蒺藜、百脉根和拟南芥。形态学和转录分析结合流式细胞术和组织化学证明了该蛋白参与植物生长和发育的许多方面，包括间接体细胞胚胎发生和共生体形成过程。细胞周期蛋白样F-box基因在所有植物器官和组织中表达，这些器官和组织由活跃的分裂细胞组成。在获得的转基因株系中观察到的根和下胚轴生长、叶和角果发育、倍性水平和叶参数的变化证明了该基因对器官发育的影响。此外，敲除细胞周期蛋白样F-box导致更高水平的G2/M转换特异性基因细胞周期蛋白B1：1（CYCB 1：1）的积累，这表明其可能在细胞周期控制中的作用。总之，收集到的数据表明，在三个模型物种中的细胞周期蛋白样F-box蛋白的类似作用，为所研究的基因的功能保守性提供了证据。
In eukaryotes, F-box proteins are one of the main components of the SCF complex that belongs to the family of ubiquitin E3 ligases, which catalyze protein ubiquitination and maintain the balance between protein synthesis and degradation. In the present study, we clarified the role and function of the gene encoding cyclin-like F-box protein from Medicago truncatula using transgenic plants of the model species M. truncatula, Lotus japonicas, and Arabidopsis thaliana generated by Agrobacterium-mediated transformation. Morphological and transcriptional analyses combined with flow cytometry and histochemistry demonstrated the participation of this protein in many aspects of plant growth and development, including processes of indirect somatic embryogenesis and symbiotic nodulation. The cyclin-like F-box gene showed expression in all plant organs and tissues comprised of actively dividing cells. The observed variations in root and hypocotyl growth, leaf and silique development, ploidy levels, and leaf parameters in the obtained transgenic lines demonstrated the effects of this gene on organ development. Furthermore, knockdown of cyclin-like F-box led to accumulation of higher levels of the G2/M transition-specific gene cyclin B1:1 (CYCB1:1), suggesting its possible role in cell cycle control. Together, the collected data suggest a similar role of the cyclin-like F-box protein in the three model species, providing evidence for the functional conservation of the studied gene.