Associated analysis between temporal and spatial expression of fiber development genes and fiber quality.
Associated analysis between temporal and spatial expression of fiber development genes and fiber quality.
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DOI：
10.3724/sp.j.1006.2009.01217
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发表时间：
2009-01-01
期刊：
影响因子：
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通讯作者：
Guo, W. Z.
中科院分区：
文献类型：
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作者：
Ju Ming, Ju Ming;Wang HaiTang, Wang HaiTang;Guo, W. Z.
本文选取了以往报道的10个在纤维发育期优先表达的基因，筛选出14个纤维品质差异明显的棉花品种（品系）。采用实时定量RT-PCR（qRT-PCR）技术，检测花后0天（DPA）、5DPA、10DPA、15DPA、20DPA、23DPA 6个不同纤维发育阶段基因的相对表达值以及14个棉花品种（品系）的纤维品质数据。表达谱显示GhExp1、GhCIPK1、GhSus1、GhSusA1和GhPL基因在纤维伸长过程中优先表达； GhACT1、GhRacA、GhRacB基因均在纤维伸长前期和次生细胞壁增厚期高表达。两个纤维素合酶基因(GhCelA1和GhCelA3)主要在纤维伸长后期和次生细胞壁增厚期表达。对于大多数基因来说，低表达期的表达值与纤维品质具有显着相关性，而除GhRacA基因外，这些基因在优先表达期没有检测到显着相关性。 14个棉花品种（品系）20DPA纤维中GhExp1的表达水平与纤维强度、整齐度呈显着负相关，与纤维伸长率呈显着正相关； 23DPA中GhPL基因的表达水平与纤维长度呈显着负相关； 5DPA和23DPA中GhRacA基因的表达水平与纤维伸长率均呈高度显着正相关； 10DPA中GhRacB基因的表达量与纤维长度、均匀度呈显着负相关； 5DPA中GhCelA1基因的表达量与纤维长度呈显着正相关，与马克隆值呈显着负相关； 10DPA中GhCelA1基因的表达量与纤维长度呈显着负相关，与马克隆值呈显着正相关，与纤维伸长率呈高度显着相关。未检测GhCIPK1、GhACT1、GhSus1、GhSusA1、GhCelA3基因表达量与纤维品质指标的相关性。
Ten genes expressed preferentially in fiber development period reported previously and 14 cotton cultivars (strains) with distinctly different fiber quality were selected in this paper. To test the relative expression values of the genes in six different fiber developmental stages, 0 day post anthesis (DPA), 5DPA, 10DPA, 15DPA, 20DPA, 23DPA, by real-time quantitative RT-PCR (qRT-PCR), and the data of fiber qualities from 14 cotton varieties (strains). The expression profile showed that GhExp1, GhCIPK1, GhSus1, GhSusA1, and GhPL genes were expressed preferentially during fiber elongation; GhACT1, GhRacA, and GhRacB genes all had high expression level in earlier stage of fiber elongation and the thickening period of secondary cell wall. Two cellulose synthase genes (GhCelA1 and GhCelA3) were expressed predominantly during late stage of fiber elongation and the thickening period of secondary cell wall. For most genes, the expression value in low expression level period had significant correlation with fiber quality, while no significant correlation was detected in preferential expression stage of these genes with an exception of GhRacA gene. The expression level of GhExp1 in 20DPA fiber of 14 cotton varieties (strains) had a significant negative correlation with fiber strength and uniformity and a significant positive correlation with fiber elongation percentage; the expression level of GhPL gene in 23DPA had a significant negative correlation with fiber length; the expression level of GhRacA gene in 5DPA and 23DPA both had a high significant positive correlation with fiber elongation percentage; the expression level of GhRacB gene in 10DPA had a significant negative correlation with fiber length and uniformity; the expression level of GhCelA1 gene in 5DPA had a significant positive correlation with fiber length and a significant negative correlation with micronaire value; the expression level of GhCelA1 gene in 10DPA had a significant negative correlation with fiber length, a significant positive correlation with micronaire value, and a high significant correlation with fiber elongation percentage; the correlation between the expression levels of GhCIPK1, GhACT1, GhSus1, GhSusA1 and GhCelA3 gene and fiber quality indexes had not been detected.