A method for intravital monitoring of human cells using a far-red luminescent probe in graft-versus-host disease model mice.
A method for intravital monitoring of human cells using a far-red luminescent probe in graft-versus-host disease model mice.
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一种在移植物抗宿主疾病模型小鼠中使用远红发光探针对人体细胞进行活体监测的方法。
DOI：
10.1038/jid.2012.345
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发表时间：
2013
影响因子：
6.5
通讯作者：
Qiao H
中科院分区：
文献类型：
--
作者：
Kazumi Kasahara;Satoshi Tanaka;Takashi Hanakawa;Atsushi Senoo;Manabu Honda;笠原和美;Shibasaki S;Qiao H
在皮肤移植区域中特异性检测到显示高信号的生物发光图像（图2b）。我们实验中的NOG小鼠是严重免疫受损的，并且几乎不能识别淋巴结（Kambe等人，2004年）。因此，仅在人皮肤移植区域观察到人淋巴细胞蓄积。在人白细胞注射后6天，没有表现，并且使用人CD45染色在其他皮肤中没有检测到人细胞（数据未显示）。免疫染色证实，在皮肤移植物中积累了大量浸润的人CD45 β细胞（图2c）。在GVHD区域的上层真皮中检测到一些鼠树突状细胞（DC）（鼠CD11细胞），而在同一区域中浸润了大量人T细胞（人CD3细胞）（在线补充图S2）。相反，在GVHD区域中存在一定数量的人DC（人CD11受体），并且一些人DC似乎位于人T细胞附近（补充图S3
Bioluminescence images showing a high signal were specifically detected in the skin-grafted areas (Figure 2b). The NOG mice in our experiment are severely immunocompromised, and lymph nodes could hardly be identified (Kambe et al., 2004). Therefore, human lymphocyte accumulation was observed only in the human skin–grafted area. Six days after human leukocyte injection, there were no manifestations and no human cells were detected in other skin using human CD45 staining (data not shown). Immunostaining confirmed that numerous infiltrating human CD45þ cells were accumulated in the skin graft (Figure 2c). A few murine dendritic cells (DCs)(murine CD11cþ) were detected in the upper dermis of the GVHD area, whereas numerous human T cells (human CD3þ) were infiltrated in the same area (Supplementary Figure S2 online). In contrast, there was a certain number of human DCs (human CD11cþ) in the GVHD area, and some human DCs seemed to locate closely to human T cells (Supplementary Figure S3