Paramagnetic Structures within a Microfluidic Channel for Enhanced Immunomagnetic Isolation and Surface Patterning of Cells.
Paramagnetic Structures within a Microfluidic Channel for Enhanced Immunomagnetic Isolation and Surface Patterning of Cells.
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DOI：
10.1038/srep29407
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发表时间：
2016-07-08
影响因子：
4.6
通讯作者：
Lu C
中科院分区：
文献类型：
--
作者：
Sun C;Hassanisaber H;Yu R;Ma S;Verbridge SS;Lu C
在本报告中，我们展示了一种将磁性结构嵌入微流体通道内以进行细胞分离的独特方法。我们使用模制工艺用钴铁氧体纳米粒子的铁磁流体制造这些结构。我们表明，嵌入式磁性结构显着增加了通道中的磁场，与没有这些嵌入式磁性结构的通道相比，免疫磁性捕获增强了高达 4 倍。我们还通过实验和计算研究了捕获细胞的空间分布。我们确定这些被捕获细胞的表面图案是由磁铁的位置和通道内磁性结构的布局决定的。我们的磁性结构嵌入式微流体装置在 4mm/s 的流速下实现了超过 90% 的捕获效率，该速度比以前用于类似目的的微流体系统快了大约两个数量级。我们设想我们的技术将为从生物样品中检测和富集稀有细胞提供强大的工具。
In this report, we demonstrate a unique method for embedding magnetic structures inside a microfluidic channel for cell isolation. We used a molding process to fabricate these structures out of a ferrofluid of cobalt ferrite nanoparticles. We show that the embedded magnetic structures significantly increased the magnetic field in the channel, resulting in up to 4-fold enhancement in immunomagnetic capture as compared with a channel without these embedded magnetic structures. We also studied the spatial distribution of trapped cells both experimentally and computationally. We determined that the surface pattern of these trapped cells was determined by both location of the magnet and layout of the in-channel magnetic structures. Our magnetic structure embedded microfluidic device achieved over 90% capture efficiency at a flow velocity of 4 mm/s, a speed that was roughly two orders of magnitude faster than previous microfluidic systems used for a similar purpose. We envision that our technology will provide a powerful tool for detection and enrichment of rare cells from biological samples.