AXIAL ARRANGEMENT OF CROSSBRIDGES IN THICK FILAMENTS OF VERTEBRATE SKELETAL-MUSCLE
AXIAL ARRANGEMENT OF CROSSBRIDGES IN THICK FILAMENTS OF VERTEBRATE SKELETAL-MUSCLE
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DOI：
10.1016/s0022-2836(76)80057-5
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发表时间：
1976-01-01
影响因子：
5.6
通讯作者：
OFFER, G
中科院分区：
文献类型：
--
作者：
CRAIG, R;OFFER, G
亚片段-1的抗体用于标记甘油化的兔腰肌，以确定横桥的轴向排列。在电子显微镜下，除了中心和末端附近，在整个A带中观察到密集的标记。如Huxley所提出的，在灯丝的中心有一个没有横桥的区域;这个裸露区约为1500埃。宽，并且除了从端部起的第二和第三横桥之间的单个间隙之外，横桥以连续阵列从裸区的边缘延伸到细丝的端部。Pepe和Squire提出的粗丝模型与这些观测结果不一致。
Antibodies to subfragment-1 were used to label glycerinated rabbit psoas muscle in order to determine the axial arrangement of the crossbridges. In the electron microscope dense labeling is observed throughout the A-band except at the center and near the ends. There is a region in the center of the filament devoid of crossbridges as Huxley proposed; this bare zone is about 1500 .ANG. wide, and the crossbridges extend in a continuous array from the edge of the bare zone to the end of the filament except for a single gap between the 2nd and 3rd crossbridges from the end. The models of the thick filament proposed by Pepe and Squire are inconsistent with these observations.