Immunolpathology of acute galactosamine hepatitis in rats
Immunolpathology of acute galactosamine hepatitis in rats
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DOI：
10.1002/hep.1840110415
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发表时间：
1990-04
期刊：
影响因子：
13.5
通讯作者：
A. Jonker;F. Dijkhuis;F. Kroese;M. J. Hardonk;J. Grond
中科院分区：
文献类型：
--
作者：
A. Jonker;F. Dijkhuis;F. Kroese;M. J. Hardonk;J. Grond
盐酸半乳糖胺会诱发肝脏疾病，其形态类似于人类药物诱发的肝炎。在这项研究中，我们使用针对淋巴细胞亚群和巨噬细胞的广泛单克隆抗体分析了施用盐酸半乳糖胺造成的毒性损伤后炎症反应的特征。用多克隆抗结蛋白抗体鉴定了脂肪储存细胞。通过用胸苷类似物溴脱氧尿苷标记 S 期细胞来评估细胞增殖。注射半乳糖胺盐酸盐与前 24 小时内明显的肝细胞坏死和实质粒细胞流入有关。此后，单核炎症细胞占主导地位，主要是T淋巴细胞和巨噬细胞，在48小时时数量最多。大多数 T 淋巴细胞是 CD8 阳性细胞，位于汇管束和实质中。 CD4 阳性 T 细胞稀少且局限于汇管束。脂肪储存细胞的增殖与肝细胞再生平行，在 48 至 72 小时后达到最大值。
Galactosamine hydrochloride induces liver disease in tats that morphologically resembles drug‐induced hepatitis in man. In this study we analyzed the character of the inflammatory reaction following the toxic damage resulting from the administration of galactosamine hydrochloride using a broad panel of mono clonal antibodies to lymphocyte subsets and macrophages. Fat‐storing cells were identified with a polyclonal anti‐desmin antibody. Cellular proliferation was assessed by labeling S‐phase cells with the thymidine analog bronmodeoxyuridine. Injection of galactosamine hydrochloride was associated with conspicuous hepatocyte necrosis and parenchymal granulocyte influx in the first 24 hr. thereafter, mononuclear inflammatory cells predominated, mainly T lymphocytes and macorphages, with maximal numbers at 48 hr. The majority of T lymphocytes were CD8‐positive cells and were located in the portal tracts and parenchyma. CD4 positive T cells were scarce and confined to the portal tracts. Proliferation of fat‐storing cells paralleled hepatocyte regeneration with maximal values after 48 to 72 hr.