BUTYRATE INCREASES COLONOCYTE PROTEIN-SYNTHESIS IN ULCERATIVE-COLITIS
BUTYRATE INCREASES COLONOCYTE PROTEIN-SYNTHESIS IN ULCERATIVE-COLITIS
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DOI：
10.1006/jsre.1994.1133
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发表时间：
1994-07-01
影响因子：
2.2
通讯作者：
ROMBEAU, J
中科院分区：
文献类型：
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作者：
FRANKEL, W;LEW, J;ROMBEAU, J
丁酸盐促进上皮细胞愈合，并改善症状时，直肠给药的远端溃疡性结肠炎(UC)患者。据推测，丁酸盐可能通过刺激结肠细胞的增殖和/或蛋白质的产生来促进UC患者的愈合。降结肠粘膜取自UC(n=5)、克罗恩病(n=8)、憩室炎(n=6)和癌(正常组织距肿瘤10 cm；n=10)。用分散酶/胶原酶和差速沉淀法分离上皮细胞，在37℃下用丁酸钠(10 MM)或氯化钠(10 MM)孵育4小时。[C-14]亮氨酸掺入法测定蛋白质合成，[H-3]胸腺嘧啶核苷测定细胞增殖。平均存活率和纯度均为88%。与憩室炎和正常对照组相比，UC患者的自发增殖显著增加。与生理盐水对照组相比，丁酸盐显著增加UC上皮细胞的蛋白质合成。丁酸盐对UC患者的治疗效果可能是由于它被上皮细胞用作代谢燃料，以增加蛋白质的产生和促进愈合。(C)1994年学术出版社。
Butyrate promotes epithelial cell healing and improves symptoms when administered rectally in patients with distal ulcerative colitis (UC). It was hypothesized that butyrate may enhance healing in patients with UC by stimulating colonocyte proliferation and/or protein production. Mucosa from the descending colon was obtained from patients with UC (n = 5), Crohn's disease (n = 8), diverticulitis (n = 6), and cancer (normal tissue 10 cm from tumor; n = 10). Epithelial cells were isolated using dispase/collagenase and differential sedimentation and incubated for 4 hr at 37 degrees C with either Na butyrate (10 mM) or NaCl (10 mM). Protein synthesis was assessed by [C-14]leucine incorporation and proliferation was determined with [H-3]thymidine. Mean viability and purity were >88%. Spontaneous proliferation was significantly increased in UC when compared to diverticulitis and normal controls. Butyrate significantly increased protein synthesis in UC epithelial cells when compared to saline control. The therapeutic effects of butyrate in patients with UC may be due to its use by epithelial cells as a metabolic fuel to increase protein production and promote healing. (C) 1994 Academic Press, Inc.