Morphogenetic and functional activity of type II cells in early fetal rhesus monkey lungs. A comparison between primates and rodents.
Morphogenetic and functional activity of type II cells in early fetal rhesus monkey lungs. A comparison between primates and rodents.
复制标题
早期胎儿恒河猴肺中 II 型细胞的形态发生和功能活性。
DOI：
10.1002/ar.1092340111
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Plopper,CG
中科院分区：
文献类型：
--
作者：
TenHave-Opbroek,AA;Plopper,CG
为进一步探讨II型肺泡上皮细胞在灵长类肺发育中的作用，在有/无α-淀粉酶预处理的情况下，用抗表面活性蛋白A（SP-A）的抗体、阿尔新蓝（AB）染色和高碘酸-希夫（PAS）染色研究了（46至155天胎龄[DGA]）、出生后和成年恒河猴。II型细胞（长方体形状;大而圆的细胞核）显示SP-A的独特胞质染色。在产前肺中，62 DGA首次检测到低柱状至立方形细胞，细胞核大而圆。这是唯一的细胞类型内衬的最远端小管或芽的未来呼吸道。其表现出（最初部分）SP-A细胞质染色。AB和PAS染色显示存在酸性糖复合物和大的顶端和/或基底糖原领域。95 DGA后，远端呼吸道衬里还显示出较平坦的细胞，具有SP-A免疫反应性和非反应区。柱状上皮（假复层或单层）从未对SP-A染色。我们得出结论，形态学上可识别的II型细胞在62 DGA（假腺期）首次出现在胎恒河猴肺中。细胞可能已经合成了表面活性剂和细胞外基质成分。它们产生I型细胞，从而产生整个肺腺泡衬里。这些关于恒河猴的结论与我们先前关于另一种灵长类动物（人类）和啮齿类动物的结论完全一致。然而，如目前所示，灵长类动物与啮齿类动物在II型细胞分化的时间方面存在很大差异（妊娠期29-38%对73-75%，或产前肺发育22-25%对48-49%）。© 1992 Wiley利斯公司
To evaluate further the role of type II alveolar epithelial cells in primate lung development, lungs of fetal (46 to 155 days gestational age [DGA]), postnatal, and adult rhesus monkeys were investigated with antibodies against surfactant protein A (SP‐A), Alcian blue (AB) staining, and periodic acid‐Schiff (PAS) staining with/without alpha‐amylase pre‐treatment.In adult and postnatal lungs, type II cells (cuboid shape; large, roundish nucleus) displayed a unique cytoplasmic staining for SP‐A. In prenatal lungs, a low‐columnar to cuboid type of cell with a large, roundish nucleus was first detectable by 62 DGA. It was the only cell type to line the distalmost tubules or buds of the prospective respiratory tract. It exhibited (initially partial) cytoplasmic staining for SP‐A. AB and PAS stainings showed the presence of acid glycoconjugates and large apical and/or basal glycogen fields. After 95 DGA, the lining of the distal respiratory tract additionally displayed flatter cells with immunoreactivity for SP‐A and non‐reactive zones. Columnar epithelium (pseudostratified or simple) never stained for SP‐A.We conclude that morphologically identifiable type II cells first appear in fetal rhesus monkey lungs by 62 DGA (pseudoglandular period). The cells may already synthesize surfactant and extracellular matrix components. They generate type I cells, and thus the entire pulmonary acinus lining. These conclusions for the rhesus monkey fully agree with our earlier conclusions for another primate, the human, and for rodents. However, as presently shown, primates differ greatly from rodents with respect to the timing of type II cell differentiation (at 29–38% versus 73–75% of gestation or at 22–25% versus 48–49% of prenatal lung development). © 1992 Wiley‐Liss, Inc.