Three dimensional poly(ε-caprolactone) and silk fibroin nanocomposite fibrous matrix for artificial dermis
Three dimensional poly(ε-caprolactone) and silk fibroin nanocomposite fibrous matrix for artificial dermis
复制标题
用于人工真皮的三维聚（ε-己内酯）-丝素蛋白纳米复合纤维基质
DOI：
10.1016/j.msec.2016.06.019
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发表时间：
2016-11-01
影响因子：
7.9
通讯作者：
Park, Chan Hum
中科院分区：
文献类型：
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作者：
Lee, Jung Min;Chae, Taesik;Park, Chan Hum
理想的真皮替代物应该具有与天然皮肤组织类似的物理化学和生物学特性。在这项研究中，我们报道了一种新的策略，将聚己内酯(PCL)和丝素蛋白(SF)控制的3D纳米复合纤维基质用于人工真皮应用。使用定制设计的冷板电纺和自动磁力搅拌系统，通过在表面修饰SF颗粒的PCL纳米纤维上积累冰晶层，获得了厚度达6 mm的PCL纳米纤维。牺牲的冰晶诱导了数十到数百PM的相互连接的大孔隙。搅拌系统引入了SF蛋白在纳米纤维内部/表面的均匀分布，防止了颗粒的沉淀和团聚。NIH3T3成纤维细胞在PCL和PCL/SF支架上体外增殖7d，但组间差异无统计学意义。相反，体内大鼠模型研究显示，伤口愈合率和胶原沉积随着纳米复合材料中SF含量的增加而增加。PCL/SF纳米复合纤维的独特三维结构为天然细胞渗透到支架中提供了理想的空间线索、表面形貌和表面化学。这种纳米复合材料的伤口愈合能力与商业Matriderm(R)人造真皮相当。(C)2016爱思唯尔B.V.保留所有权利。
Ideal dermal substitutes should have comparable physicochemical and biological properties to the natural skin tissue. In this study, we report a novel strategy to "engineer" controlled 3D nanocomposite fibrous matrix of poly(epsilon-caprolactone) (PCL) and silk fibroin (SF) for an artificial dermis application. Using a custom-designed cold-plate electrospinning and automatic magnet agitation system, up to 6 mm of the thickness was achieved resulting from the accumulation of ice crystal layers on the PCL nanofibers surface-modified with the SF particles. The sacrificed ice crystals induced interconnected macro-pores ranging from tens to hundreds pm. The agitation system introduced uniform distribution of the SF protein within/on the nanofibers, preventing the particles from precipitation and agglomeration. NIH 3T3 fibroblasts proliferated in vitro on the PCL and PCL/SF scaffolds for 7 days, but there was no statistical difference between the groups. Conversely, In vivo rat model studies revealed that the wound healing rate and collagen deposition increased with the SF content within the nanocomposites. The unique 3D construct with the PCL/SF nanocomposite fibers provided desirable spatial cues, surface topography, and surface chemistry for the native cells to infiltrate into the scaffolds. The wound healing potential of the nanocomposites was comparable to the commercial Matriderm (R) artificial dermis. (C) 2016 Elsevier B.V. All rights reserved.