Cellular uptake and cytotoxic evaluation of fullerenol in different cell lines
Cellular uptake and cytotoxic evaluation of fullerenol in different cell lines
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富勒醇在不同细胞系中的细胞摄取和细胞毒性评价
DOI：
10.1016/j.tox.2009.11.015
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发表时间：
2010-03-10
期刊：
影响因子：
4.5
通讯作者：
Huang, Qing
中科院分区：
文献类型：
--
作者：
Su, Yuanyuan;Xu, Jing-ying;Huang, Qing
本文报道了一种重要的水溶性富勒烯衍生物C-60（OH）（x）的聚集、细胞摄取和细胞毒性的研究。结果表明，C-60（OH）（x）在含血清的培养基中体积较小，但难以进入细胞; C-60（OH）（x）对中国仓鼠肺细胞和中国仓鼠卵巢细胞有较强的毒性作用，而对L929细胞几乎无毒性作用，表明C-60（OH）（x）的细胞毒性具有细胞类型特异性。流式细胞术检测C-60（OH）（x）对细胞周期的精确影响，发现细胞被阻滞在G1期。这些结果有助于理解富勒烯衍生物的毒性。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
We reported the studies of aggregation, cellular uptake and cytotoxicity of C-60(OH)(x), one of important water-soluble fullerene derivatives. The results showed C-60(OH)(x) was smaller in size but harder to go into cells in the media with serum. Moreover, C-60(OH)(x) showed severe toxicity to Chinese hamster lung and Chinese hamster ovary cells, but almost no effect on L929 cells, which implied that the cytotoxicity of C-60(OH)(x) was cell type specific. Flow cytometry was used to examine the precise effect of C-60(OH)(x) on the cell cycle and found cells was blocked at G1 phase. These results are expected to be helpful to understand the toxicity of fullerene derivatives. (C) 2009 Elsevier Ireland Ltd. All rights reserved.