Hypertensive dog plasma inhibits the Na+-K+ pump of cultured vascular smooth muscle.
Hypertensive dog plasma inhibits the Na+-K+ pump of cultured vascular smooth muscle.
复制标题
高血压狗血浆抑制培养血管平滑肌的Na-K泵。
DOI：
10.1161/01.hyp.12.1.32
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Magargal,WW
中科院分区：
文献类型：
--
作者：
Overbeck,HW;Wallick,ET;Shikuma,R;Magargal,WW
研究了肾周围性高血压犬血浆对培养犬血管平滑肌细胞Na+-K+泵的影响。我们还测量了心肌和血管组织对[3H]沃巴因的结合。在良性单肾单包裹性高血压的前6周内，将健康犬的新鲜、未经处理的血浆和配对的正常对照犬的血浆分层在融合培养的幼犬主动脉平滑肌细胞上，这些细胞已加载了莫能菌素钠。在对四对狗的26对血浆进行的配对试验中，在高血压狗的血浆中培养的细胞明显减少了86Rb+的总摄入量（p < 0.01）和瓦阿因敏感（p < 0.001），但它们的细胞内钠含量与在正常血压的血浆中培养的细胞没有差异。当传代细胞或与牛内皮细胞共培养的细胞用于检测或当血浆用蛋白酶抑制剂处理或煮沸时，我们不再检测到这些摄取差异。然而，煮沸的血浆增加了实验细胞的钠含量，这表明了一种类似离子载体的作用。在高血压的第1至6周，血浆中泵抑制活性水平似乎保持不变。我们没有发现证据表明这些高血压狗的心血管组织中泵位点的数量发生了改变。这些发现支持了血浆因子抑制血管平滑肌细胞膜Na+-K+泵的假设。这些血浆抑制因子显然不诱导泵分子。
We investigated the effects of plasma from dogs with perinephritic hypertension on the Na+-K+ pump of cultured dog vascular smooth muscle cells. We also measured [3H]ouabain binding by myocardium and vascular tissue. Fresh, unprocessed plasma from healthy dogs during the first 6 weeks of benign one-kidney, one wrapped hypertension and from paired normotensive control dogs was layered over confluent primary cultured puppy aortic smooth muscle cells that had been sodium-loaded with monensin. In 26 paired assays of plasma from four pairs of dogs, cells incubated in the presence of plasma from hypertensive dogs had significantly reduced total (p less than 0.01) and ouabain-sensitive (p less than 0.001) 86Rb+ uptakes, but their intracellular sodium content did not differ from cells incubated in paired normotensive plasma. We no longer detected these uptake differences when passaged cells or cells cocultured with bovine endothelial cells were used for assay or when plasma was treated with protease inhibitors or boiled. However, boiled plasma increased the sodium content of the assay cells, suggesting an ionophorelike effect. Levels of pump inhibitory activity in plasma appeared to remain constant during Weeks 1 to 6 of hypertension. We found no evidence for altered numbers of pump sites in cardiovascular tissues from these hypertensive dogs. These findings support the hypothesis that plasma factors inhibit the membrane Na+-K+ pump in vascular smooth muscle cells in this form of hypertension. These plasma inhibitory factors apparently do not induce pump molecules.