Transcriptome analysis of Paris polyphylla var. yunnanensis illuminates the biosynthesis and accumulation of steroidal saponins in rhizomes and leaves
Transcriptome analysis of Paris polyphylla var. yunnanensis illuminates the biosynthesis and accumulation of steroidal saponins in rhizomes and leaves
复制标题
重楼转录组分析
DOI：
10.1016/j.phytochem.2020.112460
复制
发表时间：
2020-10-01
期刊：
影响因子：
3.8
通讯作者：
Liu, Changning
中科院分区：
文献类型：
--
作者：
Gao, Xiaoyang;Zhang, Xuan;Liu, Changning
多叶巴黎变种滇重楼能合成巴黎皂苷，具有多种有效的治疗作用，其根茎已成为许多专利药物中不可或缺的成分。然而，巴黎皂苷含量在不同阶段的组织中如何变化以及生物活性化合物产生和积累的分子机制尚不清楚。本研究旨在通过转录组测序和植物化学研究相结合的方法，对不同生长阶段的叶片和根茎进行分析，以揭示其生物合成和积累的机制。结果期叶片中巴黎皂苷含量低于营养生长期，而根茎中皂苷含量则高于营养生长期。通过转录组分析确定与巴黎皂苷生物合成相关的候选基因。根据表达谱和功能富集结果，发现大多数生物合成基因在营养生长期的叶片中大量表达。与皂苷生物合成相关的差异表达基因的表达模式与皂苷在组织中的积累呈正相关。这些发现表明，叶和根茎都能够生物合成巴黎皂苷，并且气生植物部分可用于提取它们。在这里也确定了不同的模式的生物合成和积累的叶片和根茎。本研究有助于加深对巴黎皂苷生物合成和积累机制的认识，并有助于对该药用植物的综合开发利用。
Paris polyphylla var. yunnanensis can synthesize Paris saponins with multiple effective therapies, and its rhizome has become an indispensable ingredient in many patented drugs. However, how Paris saponin content changes in tissues at different stages and the molecular mechanisms underlying the production and accumulation of the bioactive compounds are unclear. This study aimed to uncover the mechanisms underlying the biosynthesis and accumulation by integrating transcriptome sequencing and phytochemical investigation of the leaves and rhizomes at different growth stages. Paris saponin content in leaves was lower during the fruiting stage than the vegetative stage, whereas the content in rhizomes increased during the fruiting stage. The candidate genes related to Paris saponin biosynthesis were determined by transcriptome analyses. Most biosynthetic genes were found to be abundantly expressed in the leaves during the vegetative stage in the light of expression profiles and functional enrichment results. The expression patterns of the differentially expressed genes related to the biosynthesis were positively correlated with the accumulation of saponins in tissues. These findings suggest that both leaves and rhizomes are capable of biosynthesizing Paris saponins, and that aerial plant parts can be used to extract them. The different patterns of biosynthesis and accumulation in the leaves and rhizomes were also determined here. This study will help improve our understanding of the mechanisms underlying the biosynthesis and accumulation of Paris saponins, and aid in the comprehensive development and utilization of this medicinal plant.