PREPARATION OF OLIGOSACCHARIDES BY THE ACTION OF BACTERIOPHAGE-BORNE ENZYMES ON KLEBSIELLA CAPSULAR POLYSACCHARIDES
PREPARATION OF OLIGOSACCHARIDES BY THE ACTION OF BACTERIOPHAGE-BORNE ENZYMES ON KLEBSIELLA CAPSULAR POLYSACCHARIDES
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DOI：
10.1016/s0008-6215(00)80530-3
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发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
STEPHEN, AM
中科院分区：
文献类型：
--
作者：
DUTTON, GGS;DIFABIO, JL;STEPHEN, AM
利用病毒酶解聚细菌荚膜多糖为制备.gtoreq对应的低聚糖提供了一种方便的方法。多糖的1个重复单位。先前用于纯化噬菌体颗粒的方法以及用于分离和纯化由噬菌体作用产生的低聚物的程序进行了修改，以便为降解产物提供更直接的途径。报道了噬菌体繁殖和这些低聚糖分离的改进技术。这些简化的方法使得使用噬菌体作为制备克级低聚糖的方便试剂成为可能。在所检测的某些多糖中存在的酸和碱不稳定取代基似乎不受解聚条件的影响。通过对克雷伯菌K17、K36、K46、K60、K63和K74血清型荚膜多糖的降解，验证了该方法的可行性。
Depolymerization of bacterial capsular polysaccharides by viral enzymes provides a convenient method for preparing oligosaccharides that correspond to .gtoreq. 1 repeating unit(s) of the polysaccharide. Previous methods used for purifying bacteriophage particles and the procedures employed in the isolation and purification of the oligomers generated by the bacteriophage action were so modified as to provide a more direct route to the degradation products. Improved techniques for the propagation of bacteriophage and for the isolation of these oligosaccharides are reported. These simplified methods make possible the use of bacteriophages as convenient reagents for the preparation of oligosaccharides on a gram scale. The acid- and base-labile substituents present in certain of the polysaccharides examined were seemingly unaffected by the conditions used for depolymerization. The methods are illustrated by degradation of the capsular polysaccharides from Klebsiella serotypes K17, K36, K46, K60, K63 and K74.