Direct measurement of inositol in bovine myelin basic protein.
Direct measurement of inositol in bovine myelin basic protein.
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直接测定牛髓磷脂碱性蛋白中的肌醇。
DOI：
10.1042/bj2480285
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Sherman,WR
中科院分区：
文献类型：
--
作者：
Smith,R;Braun,PE;Ferguson,MA;Low,MG;Sherman,WR
用四种方法从牛中枢神经系统髓鞘中分离出髓鞘碱性蛋白，但没有一种方法将蛋白质暴露于酸。纯化后，水解和未水解蛋白质的肌醇含量通过g.c.- M.S.通过所有方法制备的碱性蛋白含有小于4mol%的肌醇。它的结论是，与以前的建议相反，共价结合到phosphoinositides并不代表一个一般的机制，这种细胞质取向的蛋白质连接到其膜。
Myelin basic protein has been isolated from bovine central-nervous-system myelin by four methods, none of which exposes the protein to acid. After purification the inositol content of both hydrolysed and unhydrolysed protein was quantified by g.c.-m.s. Basic protein prepared by all methods contained less than 4 mol % of inositol. It is concluded, contrary to a previous proposal, that covalent binding to phosphoinositides does not represent a general mechanism for attachment of this cytoplasmically-oriented protein to its membrane.