Switching cultivation for enhancing biomass and lipid production with extracellular polymeric substance as co-products in Heynigia riparia SX01

Switching cultivation for enhancing biomass and lipid production with extracellular polymeric substance as co-products in Heynigia riparia SX01
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Heynigia riparia SX01 中以细胞外聚合物为副产品的转耕方式提高生物量和脂质产量

DOI:
10.1016/j.biortech.2016.12.039
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发表时间:
2017-03-01
影响因子:
11.4
通讯作者:
Miao, Xiaoling
Miao, Xiaoling
中科院分区:
工程技术1区
文献类型:
--
作者:
Liu, Guodong;Miao, Xiaoling

文献摘要

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以河岸黑蝇(Heynigia riparia SX01)为研究材料,采用混合-异养,12 h: 12 h的切换培养方法,培养8 d后,最大生物量为3.55 g L-1,最大脂质产量为1.45 g L-1。胞外聚合物(EPS)作为副产物被开发出来。添加MgSO4可以提高EPS的产量。当MgSO4添加量为2 g L-1时,EPS用量最高,为0.60 g L-1,自絮凝效率高达83%。总脂质和脂质组分与添加MgSO4没有差异。在此基础上,提出了一种新的生物柴油生产模式:采用切换培养的方式,以额外的2 g L-1 MgSO4培养Heynigia riparia SX01,采用自絮凝法收集微藻用于生产生物柴油,而EPS作为有价值的副产物被收集。(C) 2016年Elsevier Ltd.出版
Switching cultivation (mixotrophic-heterotrophic, 12 h: 12 h) of Heynigia riparia SX01 was studied, the maximum biomass concentration of 3.55 g L-1 and lipid yield of 1.45 g L-1 were achieved after 8 days cultivation. The extracellular polymeric substance (EPS) was developed as co-product. Addition of MgSO4 could enhance the production of EPS. The highest amount of 0.60 g L-1 EPS was obtained with the addition of 2 g L-1 MgSO4, the self-flocculation efficiency was as high as 83% at this condition. The total lipid and lipid fractions did not show differences with extra MgSO4. Based on the above results, a new biodiesel production model was proposed: culturing Heynigia riparia SX01 with extra 2 g L-1 MgSO4 by switching cultivation and using self flocculation to collect microalgae for biodiesel production, while EPS was collected as valuable co-products. (C) 2016 Published by Elsevier Ltd.