Competitive indirect ELISA for the determination of parvalbumins from various fish species in food grade fish gelatins and isinglass with PARV-19 anti-parvalbumin antibodies.
Competitive indirect ELISA for the determination of parvalbumins from various fish species in food grade fish gelatins and isinglass with PARV-19 anti-parvalbumin antibodies.
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DOI：
10.1021/jf902470e
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发表时间：
2009-11
影响因子：
6.1
通讯作者：
P. Weber;H. Steinhart;A. Paschke
中科院分区：
文献类型：
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作者：
P. Weber;H. Steinhart;A. Paschke
小鱼蛋白是众所周知的主要鱼类过敏原。然而，目前还没有一种定量分析方法可用于测定不同鱼类中细小蛋白的含量。本研究的目的是应用凝胶层析和透析分离各种小鱼清蛋白，并开发和验证一种具有竞争力的间接ELISA法测定各种鱼类小鱼清蛋白。该方法应用于食品生产中几种鱼明胶和鱼胶样品的检测。竞争性ELISA能检测出0.1 ~ 0.5 mg/L范围内的所有小白蛋白。在任何被调查的鱼明胶或用作鱼明胶生产原料的鱼皮中均未检测到细小蛋白。相反，鱼胶含有的小白蛋白含量高达414.7 +/- 30.6 mg/kg。
Parvalbumins are well-known as major fish allergens. However, no quantitative analytical method is currently available for the determination of parvalbumins from different fish species. The aim of this study was the isolation of the various parvalbumins by the application of gel chromatography and dialysis and the development and validation of a competitive indirect ELISA for the determination of parvalbumins from various fish species. This ELISA method was applied to several fish gelatins and isinglass samples used in food production. The competitive ELISA was capable of detecting all tested parvalbumins within a range of 0.1-0.5 mg/L. No parvalbumin was detected in any of the investigated fish gelatins or in a fish skin used as raw material for fish gelatin production. Contrarily, isinglass was found to contain parvalbumin amounts of up to 414.7 +/- 30.6 mg/kg.