Characterization of cell-type specific profiles in tissues and isolated cells from squamous cell carcinomas of the lung
Characterization of cell-type specific profiles in tissues and isolated cells from squamous cell carcinomas of the lung
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DOI：
10.1016/j.lungcan.2006.04.015
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发表时间：
2006-08-01
期刊：
影响因子：
5.3
通讯作者：
Baumann, Heinz
中科院分区：
文献类型：
--
作者：
Hawthorn, Lesleyann;Stein, Leighton;Baumann, Heinz
肺癌占所有癌症死亡人数的 28%，比任何其他人类癌症都要高。鳞状细胞癌（SqCC）是最常见的肺部肿瘤，也是一种与吸烟密切相关的肿瘤。尽管许多基因改变与肺癌有关，但肿瘤发生的精确分子机制在很大程度上仍然不明确。尽管许多关于肺癌的研究已经在全球范围内使用。尽管转录获利方法旨在发现在肺部肿瘤发生中重要的基因或途径，但尚未出现强有力的候选者。这些不同研究之间的一致性在很大程度上可归因于桐组织内的细胞异质性。我们试图通过设计一种盈利策略来减少这种并发症，该策略将最大限度地减少组织异质性对肺部肿瘤基因表达的影响。具体而言，我们获得了转录本在分离细胞和组织中的表达水平。 SqCC 和正常样本。我们的策略包括组合和减去这些不同细胞类型的输入，从而产生鳞状癌细胞的独特转录谱。然后，我们使用 Pathways Assist 分析软件分析数据，以确定哪些过程可能参与 SqCC 肿瘤发生。参与生长和分化的 MAP/ERK 通路是所有比较中最常识别的通路。此外，SqCC 概况的生物相互作用网络确定 IL-8 在 SqCC 发展中发挥着潜在的重要作用。 (C) 2006 Elsevier Ireland Ltd. 保留所有权利。
Lung cancer accounts for 28% of all cancer deaths, a higher percentage than any other human cancer. Squamous Cell Carcinoma (SqCC) is the most common lung neoplasm and is a tumor that is extensively associated with tobacco use. Despite the association of many genetic alterations with lung cancer, the precise molecular mechanisms of tumorigenesis, for the most part, remain ambiguous. Although many studies of tung cancer have used global. transcript profiting approaches designed to uncover genes or pathways that are important in lung tumorigenesis, no strong candidates have emerged. A tack of concurrence amongst these various studies can be attributed, in a large part, to the cellular heterogeneity within tung tissue. We have attempted to reduce this complication by designing a profiting strategy that will minimize the confounding involvement of tissue heterogeneity in gene expression of lung tumors. expression levels in both isolated cells and tissues from Specifically, we have profited transcript. SqCC and normal samples. Our strategy consists of combining and subtracting the input of these various cell types which has produced a unique transcript profile of the squamous carcinoma cell. We then analyzed the data using Pathways Assist analysis software to determine which processes may be involved in SqCC tumorigenesis. The MAP/ERK pathway involved in growth and differentiation was the pathway that was most frequently identified across all comparisons. In addition, biological interaction networks of the SqCC profile identified IL-8 as playing a potentially important role SqCC development. (C) 2006 Elsevier Ireland Ltd. All rights reserved.