Repetitive transpositions of mitochondrial DNA sequences to the nucleus during the radiation of horseshoe bats (Rhinolophus, Chiroptera)
Repetitive transpositions of mitochondrial DNA sequences to the nucleus during the radiation of horseshoe bats (Rhinolophus, Chiroptera)
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马蹄蝠（菊头蝠、翼手目）辐射过程中线粒体 DNA 序列重复转座至细胞核
DOI：
10.1016/j.gene.2016.01.035
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发表时间：
2016
期刊：
影响因子：
3.5
通讯作者：
Mao Xiuguang
中科院分区：
文献类型：
--
作者：
Shi Huizhen;Dong Ji;Irwin David M;Zhang Shuyi;Mao Xiuguang
在真核生物中，线粒体DNA转座到细胞核中，产生核线粒体DNA（NUMT），这已被充分证明。然而，很少有研究评估了这些换位的频率在一个特定的分类组的进化历史。在这里，我们使用的马蹄蝙蝠（菊头蝠）作为案例研究，以确定线粒体控制区序列的核转移的频率和相对时间。为此，系统发育和合并分析NUMTs和真实的mtDNA序列产生的八个马蹄蝙蝠物种。我们的研究结果表明，至少有三个独立的换位，包括两个古老的和一个最近的，在进化史ofRhinolophus。这两个古老的易位由NUMT-1和NUMT-2进化枝代表，每个进化枝由几乎所有研究物种的NUMT组成，但起源于mtDNA基因组的不同部分。此外，估计最近的共同祖先为每个分支对应的时间本属的初始多样化。最近的转座是NUMT-3的代表，它只在菊头蝠的一个特定亚群中发现，并表现出与线粒体对应物的密切关系。我们在R. ferrumequinumgenome证实了NUMT-1和NUMT-2进化枝序列的存在，并首次评估了NUMT在蝙蝠基因组中的程度。据我们所知，这是第一项报告在属的共同祖先之前发生的线粒体DNA转座频率的研究。
Transposition of mitochondrial DNA into the nucleus, which gives rise to nuclear mitochondrial DNAs (NUMTs), has been well documented in eukaryotes. However, very few studies have assessed the frequency of these transpositions during the evolutionary history of a specific taxonomic group. Here we used the horseshoe bats (Rhinolophus) as a case study to determine the frequency and relative timing of nuclear transfers of mitochondrial control region sequences. For this, phylogenetic and coalescent analyzes were performed on NUMTs and authentic mtDNA sequences generated from eight horseshoe bat species. Our results suggest at least three independent transpositions, including two ancient and one more recent, during the evolutionary history ofRhinolophus. The two ancient transpositions are represented by the NUMT-1 and -2 clades, with each clade consisting of NUMTs from almost all studied species but originating from different portions of the mtDNA genome. Furthermore, estimates of the most recent common ancestor for each clade corresponded to the time of the initial diversification of this genus. The recent transposition is represented by NUMT-3, which was discovered only in a specific subgroup ofRhinolophusand exhibited a close relationship to its mitochondrial counterpart. Our similarity searches of mtDNA in theR. ferrumequinumgenome confirmed the presence of NUMT-1 and NUMT-2 clade sequences and, for the first time, assessed the extent of NUMTs in a bat genome. To our knowledge, this is the first study to report on the frequency of transpositions of mtDNA occurring before the common ancestry of a genus.