NUCLEAR FACTOR Y transcription factors have both opposing and additive roles in ABA-mediated seed germination.
NUCLEAR FACTOR Y transcription factors have both opposing and additive roles in ABA-mediated seed germination.
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DOI：
10.1371/journal.pone.0059481
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Holt BF 3rd
中科院分区：
文献类型：
--
作者：
Kumimoto RW;Siriwardana CL;Gayler KK;Risinger JR;Siefers N;Holt BF 3rd
在模式生物拟南芥（Arabidopsis thaliana）中，异源三聚体转录因子核因子Y（NF-Y）已被证明在促进植物生长和发育中发挥多种作用。虽然NF-Y本身代表多蛋白质转录复合物，但最近的研究表明与其他转录因子，特别是bZIP家族中的转录因子具有重要的相互作用。在这里，我们增加了越来越多的证据表明，NF-Y和bZIP形成共同的复合物，影响许多过程。我们对nf-yc突变体进行了转录谱分析，并通过随后的分析发现，在失调基因的启动子元件中富集了bZIP结合位点。使用NF-Y作为诱饵，酵母双杂交测定产生了与已知控制阿坝信号传导的bZIP蛋白的相互作用。因此，我们发现，植物的几个NF-Y亚基突变体表现出与阿坝信号中断的特征表型。虽然以前的报道已经显示了NF-YC家族成员在光周期开花中的附加作用，但我们发现它们在阿坝信号传导中可能具有相反的作用。总的来说，这些结果证明了NF-Y在环境和激素信号整合中的重要性和复杂性。
In the model organism Arabidopsis thaliana the heterotrimeric transcription factor NUCLEAR FACTOR Y (NF-Y) has been shown to play multiple roles in facilitating plant growth and development. Although NF-Y itself represents a multi-protein transcriptional complex, recent studies have shown important interactions with other transcription factors, especially those in the bZIP family. Here we add to the growing evidence that NF-Y and bZIP form common complexes to affect many processes. We carried out transcriptional profiling on nf-yc mutants and through subsequent analyses found an enrichment of bZIP binding sites in the promoter elements of misregulated genes. Using NF-Y as bait, yeast two hybrid assays yielded interactions with bZIP proteins that are known to control ABA signaling. Accordingly, we find that plants mutant for several NF-Y subunits show characteristic phenotypes associated with the disruption of ABA signaling. While previous reports have shown additive roles for NF-YC family members in photoperiodic flowering, we found that they can have opposing roles in ABA signaling. Collectively, these results demonstrated the importance and complexity of NF-Y in the integration of environmental and hormone signals.
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影响因子：
3.7
作者：
Calvenzani V;Testoni B;Gusmaroli G;Lorenzo M;Gnesutta N;Petroni K;Mantovani R;Tonelli C
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影响因子：
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